Impacts of high-intensity exercise on the metabolomics profile of human skeletal muscle tissue

Autor(es)

Zagatto, Alessandro M. ^[1] ; Bishop, David J. ^[2] ; Antunes, Barbara Moura ^[1] ; Beck, Wladimir R. ^[3] ; Malta, Elvis S. ^[1] ; de Poli, Rodrigo A. B. ^[1] ; Cavaglieri, Claudia R. ^[4] ; Chacon-Mikahil, Mara Patricia T. ^[4] ; Castro, Alex ^[4]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Lab Physiol & Sport Performance LAFIDE, Bauru, SP - Brazil

^[2] Victoria Univ, Inst Hlth & Sport iHeS, Footscray, Vic - Australia

^[3] Fed Univ Sao Carlos UFSCar, Sao Carlos - Brazil

^[4] Univ Campinas UNICAMP, Fac Phys Educ, Exercise Physiol Lab, Campinas - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS; v. 32, n. 2 NOV 2021.
• Citações Web of Science: 0

Resumo

The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high-intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by H-1-NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 +/- 35.5 s whereas the muscle pH at exhaustion was 6.48 +/- 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2-oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O-acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2-oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (-70.2%), ADP (-56.5%), carnitine (-33.5%), and glutamate (-42.3%). Only the succinate was significantly correlated with tlim (r = -0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high-intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim. (AU)

• Processo FAPESP: 20/13939-7 - Análise metabolômica por ressonância magnética nuclear (RMN) e espectrometria de massas (LC-MS) de amostras sanguíneas de indivíduos submetidos à reabilitação cardiorrespiratória.
• Beneficiário: Alex Castro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 17/21724-8 - Impacto do uso regular de imersão em água fria após sessões de treinamento intervalado de alta intensidade sobre o reparo tecidual, adaptação muscular e desempenho em corrida
• Beneficiário: Elvis de Souza Malta
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/25282-5 - Suplementação de b-alanina associado ao treinamento intermitente de alta intensidade sobre as modulações da resposta imune-endócrina de adultos saudáveis
• Beneficiário: Barbara de Moura Mello Antunes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/21126-6 - Fadiga no exercício supramáximo: Análise da influência de mecanismos central e periférico, acidose muscular, substrato energético, atividade enzimática e metabólitos
• Beneficiário: Alessandro Moura Zagatto
• Modalidade de apoio: Auxílio à Pesquisa - Regular