Impacts of high-intensity exercise on the metabolomics profile of human skeletal muscle tissue

Author(s)

Zagatto, Alessandro M. ^[1] ; Bishop, David J. ^[2] ; Antunes, Barbara Moura ^[1] ; Beck, Wladimir R. ^[3] ; Malta, Elvis S. ^[1] ; de Poli, Rodrigo A. B. ^[1] ; Cavaglieri, Claudia R. ^[4] ; Chacon-Mikahil, Mara Patricia T. ^[4] ; Castro, Alex ^[4]

Affiliation

^[1] Sao Paulo State Univ UNESP, Lab Physiol & Sport Performance LAFIDE, Bauru, SP - Brazil

^[2] Victoria Univ, Inst Hlth & Sport iHeS, Footscray, Vic - Australia

^[3] Fed Univ Sao Carlos UFSCar, Sao Carlos - Brazil

^[4] Univ Campinas UNICAMP, Fac Phys Educ, Exercise Physiol Lab, Campinas - Brazil

• Document type: Journal article
• Source: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS; v. 32, n. 2 NOV 2021.
• Web of Science Citations: 0

Abstract

The study aimed to identify and quantify the metabolites profile and metabolic pathways in human muscle tissue engaged during exhaustive high-intensity cycling exercise. Seven healthy physically active men performed a graded exercise test and an exhaustive supramaximal effort at 115% of maximal aerobic power with muscles biopsies performed in rest and immediately after exhaustion for quantifying of muscle metabolites changes by H-1-NMR spectroscopy. The time until exhaustion (tlim) recorded was 224.7 +/- 35.5 s whereas the muscle pH at exhaustion was 6.48 +/- 0.05. A total of 54 metabolites were identified and quantified. The most enriched and impacted pathways included: beta oxidation of very long chain fatty acids, mitochondrial electron transport chain, alanine aspartate, and glutamate metabolism, citric acid cycle, arginine biosynthesis, propanoate metabolism, threonine and 2-oxobutanoate degradation and pyruvate metabolism. In addition, the muscle concentrations in Post exercise, compared to Pre increased significantly (p < 0.0398) for fumarate (42.0%), succinate (101.2%), glucose (249.7%), lactate (122.8%), O-acetylcarnitine (164.7%), glycerol (79.3%), AMP (288.2%), 2-oxobutyrate (121.0%), and methanol (58.5%), whereas decreased significantly (p < 0.010) for creatine phosphate (-70.2%), ADP (-56.5%), carnitine (-33.5%), and glutamate (-42.3%). Only the succinate was significantly correlated with tlim (r = -0.76; p = 0.0497). Besides the classical expected contribution of glycolytic and phosphagen energetic pathways, it was demonstrated that the high-intensity exercise is also associated with pathways indicatives of amino acid and fatty acid oxidation metabolisms, highlighting the inverse relation between changes in the intramuscular succinate levels and tlim. (AU)

• FAPESP's process: 20/13939-7 - Metabolomic analysis by Nuclear Magnetic Resonance (NMR) and mass spectrometry (LC-MS) of blood samples from individuals submitted to cardiorespiratory rehabilitation.
• Grantee: Alex Castro
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/21724-8 - Impact of cold-water immersion regular use after high-intensity interval training sessions on muscle repair, muscle adaptation and running performance
• Grantee: Elvis de Souza Malta
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/25282-5 - B-alanine supplementation associated with high-intensity interval training on modulations of the immune-endocrine response in healthy adults
• Grantee: Barbara de Moura Mello Antunes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/21126-6 - Fatigue process during supramaximal effort: Analysis of central and peripherical mechanisms, muscular acidosis, energetic substrate, enzymatic activity and metabolic
• Grantee: Alessandro Moura Zagatto
• Support Opportunities: Regular Research Grants