Metabolomic analysis by Nuclear Magnetic Resonance (NMR) and mass spectrometry (LC-MS) of blood samples from individuals submitted to cardiorespiratory rehabilitation.

• Grant number: 20/13939-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: December 01, 2020
• End date: September 30, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Antonio Gilberto Ferreira
• Grantee: Alex Castro
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• Associated research grant: 16/22215-7 - Impact of inspiratory muscle training and aging on metabolic mapping, autonomic modulation, and cardiovascular, respiratory and metabolic responses, and prediction of cardiorespiratory health through wearables, AP.TEM

Abstract

NMR and MS techniques have proven to be very useful analytical tools, especially in the last decade, in the metabolomic/metabonomic study of animal and plant organisms. The combination of these orthogonal techniques allows the analysis of matrices looking for target or non-target compounds and obtaining information on the molecular structure of the compounds involved in their discrimination. In addition, quantitative results can be obtained with a minimum sample preparation time. On the other hand, the positive results generated by the use of inspiratory muscle training (IMT) in active and non-active healthy individuals have made this type of intervention one of the most studied in the last decades. IMT has already been used as an integral part of cardiorespiratory rehabilitation in individuals with respiratory muscle weakness and currently appears as an alternative to improve the athletic performance of amateur and elite athletes by reducing the sensation of dyspnea in these individuals. In addition, regular physical activity is one of the main tools for healthy aging, so the metabolic characterization of active and sedentary aging can elucidate metabolic changes during the aging process.

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE FAVARI SIGNINI, ETORE; CASTRO, ALEX; REHDER-SANTOS, PATRICIA; CRISTINA MILLAN-MATTOS, JULIANA; MAGALHAES DE OLIVEIRA, JULIANA; MINATEL, VINICIUS; BIANCA FALASCO PANTONI, CAMILA; SOBREIRO SELISTRE DE ARAUJO, HELOISA; FABRIZZI, FERNANDO; PORTA, ALBERTO; et al. Integrative perspective of the healthy aging process considering the metabolome, cardiac autonomic modulation and cardiorespiratory fitness evaluated in age groups. SCIENTIFIC REPORTS, v. 12, n. 1, p. 12-pg., 2022-12-09. (10/52070-4, 20/13939-7, 16/22215-7, 19/15040-4, 20/05965-8, 18/25082-3)

DE SANTANA, DAVI ALVES; CASTRO, ALEX; CAVAGLIERI, CLAUDIA REGINA. Strength Training Volume to Increase Muscle Mass Responsiveness in Older Individuals: Weekly Sets Based Approach. FRONTIERS IN PHYSIOLOGY, v. 12, p. 6-pg., 2021-09-30. (20/13939-7)

SARDELI, AMANDA V.; CASTRO, ALEX; GADELHA, VICTOR B.; DOS SANTOS, WELLINGTON M.; LORD, JANET M.; CAVAGLIERI, CLAUDIA R.; CHACON-MIKAHIL, MARA PATRICIA T.. Metabolomic Response throughout 16 Weeks of Combined Aerobic and Resistance Exercise Training in Older Women with Metabolic Syndrome. METABOLITES, v. 12, n. 11, p. 15-pg., 2022-11-01. (20/13939-7)

DE OLIVEIRA-NUNES, SILAS GABRIEL; CASTRO, ALEX; SARDELI, AMANDA VEIGA; CAVAGLIERI, CLAUDIA REGINA; CHACON-MIKAHIL, MARA PATRICIA TRAINA. HIIT vs. SIT: What Is the Better to Improve VO(2)max? A Systematic Review and Meta-Analysis. INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, v. 18, n. 24, DEC 2021. (20/13939-7)

ZAGATTO, ALESSANDRO M.; BISHOP, DAVID J.; ANTUNES, BARBARA MOURA; BECK, WLADIMIR R.; MALTA, ELVIS S.; DE POLI, RODRIGO A. B.; CAVAGLIERI, CLAUDIA R.; CHACON-MIKAHIL, MARA PATRICIA T.; CASTRO, ALEX. Impacts of high-intensity exercise on the metabolomics profile of human skeletal muscle tissue. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 32, n. 2, NOV 2021. (20/13939-7, 17/21724-8, 19/25282-5, 18/21126-6)

CASTRO, ALEX; DUFT, RENATA G.; DE OLIVEIRA-NUNES, SILAS GABRIEL; DE ANDRADE, ANDRE L. L.; CAVAGLIERI, CLAUDIA R.; TRAINA CHACON-MIKAHIL, MARA PATRICIA. Association Between Changes in Serum and Skeletal Muscle Metabolomics Profile With Maximum Power Output Gains in Response to Different Aerobic Training Programs: The Times Study. FRONTIERS IN PHYSIOLOGY, v. 12, OCT 20 2021. (20/13939-7)

LUPORINI, RAFAEL LUIS; POTT-JUNIOR, HENRIQUE; LEAL, MARIA CAROLINA B. DI MEDEIROS; CASTRO, ALEX; FERREIRA, ANTONIO GILBERTO; COMINETTI, MARCIA REGINA; ANIBAL, FERNANDA DE FREITAS. Phenylalanine and COVID-19: Tracking disease severity markers. International Immunopharmacology, v. 101, n. A, DEC 2021. (20/13939-7, 14/50867-3, 19/16135-9)

SILVA, ANDRESSA KUHNEN; OLIVEIRA, GERLON DE ALMEIDA RIBEIRO; CASTRO, ALEX; PRADO, CRISTIANO SALES; LIAO, LUCIANO MORAIS. The most consumed beef cuts in Brazil: Prices versus metabolic profile. Journal of Food Composition and Analysis, v. 115, p. 10-pg., 2023-01-01. (20/13939-7)

CASTRO, ALEX; SIGNINI, ETORE F.; DE OLIVEIRA, JULIANA MAGALHAES; DI MEDEIROS LEAL, MARIA CAROLINA BEZERRA; REHDER-SANTOS, PATRICIA; MILLAN-MATTOS, JULIANA C.; MINATEL, VINICIUS; PANTONI, CAMILA B. F.; OLIVEIRA, REGINA V.; CATAI, APARECIDA M.; et al. The Aging Process: A Metabolomics Perspective. Molecules, v. 27, n. 24, p. 16-pg., 2022-12-01. (16/22215-7, 19/15040-4, 10/52070-4, 20/05965-8, 20/13939-7, 18/25082-3)

CASTRO, A.; SIGNINI, E. F.; REHDER-SANTOS, P.; MILLAN-MATTOS, J. C.; DE OLIVEIRA, J. M.; MINATEL, V.; PANTONI, C. B. F.; OLIVEIRA, R. V.; CATAI, A. M.; FERREIRA, A. G.. The Aging Process: A Metabolomics Perspective. ACTA PHYSIOLOGICA, v. 236, p. 2-pg., 2022-09-01. (20/13939-7, 16/22215-7)

VALERIO, DENIS F. F.; CASTRO, ALEX; GASPARI, ARTHUR; BARROSO, RENATO. Serum Metabolites Associated with Muscle Hypertrophy after 8 Weeks of High- and Low-Load Resistance Training. METABOLITES, v. 13, n. 3, p. 17-pg., 2023-03-01. (20/13939-7)