Effects of Qualea grandiflora Extract on the Expression of MMP-14 and HIF-1α in Cultured Fibroblasts and Preosteoblasts

Autor(es)

Gabriela Silva Neubern de Oliveira ^[1] ; Cintia Kazuko Tokuhara ^[2] ; Flávia Amadeu de Oliveira ^[3] ; Márcia Sirlene Zardin Graeff ^[4] ; Zohaib Nisar Khan ^[5] ; Luiz Leonardo Saldanha ^[6] ; Anne Lígia Dokkedal ^[7] ; Rodrigo Cardoso de Oliveira ^[8]

Afiliação do(s) autor(es)

^[1] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[2] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[3] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[4] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[5] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

^[6] São Paulo State University. Faculty of Sciences. Department of Biological Sciences - Brasil

^[7] São Paulo State University. Faculty of Sciences. Department of Biological Sciences - Brasil

^[8] University of São Paulo. Bauru School of Dentistry. Department of Biological Sciences - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 64, 2021-11-19.

Resumo

Abstract Background: Qualea grandiflora (QG) (Vochysiaceae), also known as "pau-ferro", "pau-terra" or "pau-de-tucano", is a very common deciduous tree in the Brazilian Cerrado used in traditional medicine to treat inflammations, ulcers, diarrhea, and infections. There are reports in the scientific literature that demonstrate the medicinal effects of the bark and leaf of the QG. However, studies involving this plant are rather imited. Aim of the study: To perform the phytochemical analysis of the QG hydroalcoholic extract (HAE) of leaves, and to investigate it effects on fibroblast and preosteoblasts. Methods: Phytochemical analysis was done by HPLC-DAD. Murine NIH/3T3 fibroblasts and MC3T3-E1 preosteoblasts cell lines (ATCC) were used for the experiments. Cell viability was assessed by the MTT colorimetric assay and the expression of MMP-14 and HIF-1α by immunofluorescence. Results and conclusion: The following compounds were identified by HPLC-DAD, such as quinic acid, ethyl galate, ellagic acid derivatives as O-methylellagic acid O-galloyl, O-methylellagic acid O-deoxyhexoside, galloyl derivatives, flavonol glycoside as kaempferol-O-deoxyhexoside, quercetin-O-deoxyhexoside, myricetin-O-deoxyhexoside and the pentacyclic triterpene arjunglucoside. Cell viability results demonstrated no cytotoxic effects in the studied concentrations. We found in QG HAE some compounds with therapeutic properties that can increase the expression of MMP-14 and HIF-1α, in fibroblasts and preosteoblasts. These data suggest that QG HAE has an action on these two molecules widely involved in physiological conditions, such as collagen remodeling, bone development and growth and pathological processes as HIF signaling in cancer metastasis. (AU)

• Processo FAPESP: 14/20656-0 - Avaliação do efeito da Qualea grandiflora Mart na modulação de HIF-1 alpha e MMP-14 em linhagens celulares de fibroblastos e pré-osteoblastos
• Beneficiário: Gabriela Silva Neubern de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/05234-2 - Estudo do extrato hidroalcoólico de folhas de Qualea grandiflora: avaliação fitoquímica e efeitos na expressão de proteínas envolvidas em processos inflamatórios
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular