limate change affects cell-wall structure and hydrolytic performance of a perennial grass as an energy cro

Autor(es)

de Freitas, Emanuelle N. ^{[1, 2]} ; Khatri, Vinay ^[2] ; Contin, Daniele R. ^[3] ; de Oliveira, Tassio B. ^[3] ; Contato, Alex G. ^[1] ; Peralta, Rosane M. ^[4] ; dos Santos, Wanderley D. ^[4] ; Martinez, Carlos A. ^[3] ; Saddler, Jack N. ^[2] ; Polizeli, Maria de Lourdes T. M. ^{[1, 3]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biochem & Immunol, FMRP, Sao Paulo - Brazil

^[2] Univ British Columbia, Fac Forestry, Dept Wood Sci, Vancouver, BC - Canada

^[3] Univ Sao Paulo, Dept Biol, FFCLRP, Sao Paulo - Brazil

^[4] Univ Estadual Maringa, Dept Biochem, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR; v. 16, n. 2 NOV 2021.
• Citações Web of Science: 0

Resumo

Perennial grasses, such as Panicum maximum, are important alternatives to dedicated energy crops for bioethanol production. This study investigates whether future climate conditions could influence P. maximum cell-wall structure and hydrolytic performance. To analyze interactions with environmental factors in field conditions, a combined Free-air Temperature and CO2 Controlled Enhancement (Trop-T-FACE) facility was used to investigate the isolated and combined effect of elevated atmospheric CO2 concentration (eC) (600 mu mol.mol(-1)) and elevated temperature (eT) by 2 degrees C more than the ambient temperature, on cell-wall composition, cellulose crystallinity, accessibility, and hydrolysis yields. The elevated temperature treatments (eT and eT + eC) exhibited the most pronounced effects on the P. maximum cell wall. Warming reduced the starch content and the crystallinity index (CI) of cellulose and increased the cellulose content. Fluorescent protein-tagged carbohydrate-binding modules analysis demonstrated that warming improved total cellulose surface exposure/accessibility in eT and eT + eC by 181% and 132%, respectively. Consequently, glucan conversion yields were improved by 7.07% and 5.37%, showing that warming led to lower recalcitrance in P. maximum biomass, which positively affects its use in biorefineries. This work therefore provides important information from an ecological and economic point of view, allowing us to understand the mitigation process applied by this forage grass under future climate conditions. It might assist in selecting tropical forage grasses that are efficiently adapted to climate change, with a positive effect on bioenergy production. (c) 2021 Society of Chemical Industry and John Wiley \& Sons, Ltd (AU)

• Processo FAPESP: 08/58075-8 - Miniface climate-change impact experiment to analyze the effects of elevated CO2 and warming on photosynthesis, gene expression, biochemistry, growth, nutrient dynamics and yield of two contrasting tropical forage species
• Beneficiário: Carlos Alberto Martinez y Huaman
• Modalidade de apoio: Auxílio à Pesquisa - Programa de Pesquisa sobre Mudanças Climáticas Globais - Temático
• Processo FAPESP: 08/57908-6 - Instituto Nacional de Biotecnologia para o Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 17/23989-9 - Biomassa lignocelulósica da gramínea forrageira Panicum maximum Jacq. produzida em condições simuladas de clima futuro: potencial fonte de açúcares fermentescíveis para bioenergia
• Beneficiário: Emanuelle Neiverth de Freitas
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 19/07141-5 - Formulação de um coquetel enzimático sinérgico otimizado com enzimas acessórias para melhorar o desempenho hidrolítico do Panicum maximum Jacq.
• Beneficiário: Emanuelle Neiverth de Freitas
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 18/07522-6 - Desenvolvimento de tecnologias ecológicas para a hidrólise de biomassa e resíduos da indústria de celulose e papel
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Regular