Efficiency of eDNA and iDNA in assessing vertebrate diversity and its abundance

Autor(es)

Carvalho, Carolina S. ^{[1, 2]} ; De Oliveira, Marina Elisa ^[1] ; Rodriguez-Castro, Karen Giselle ^{[1, 3]} ; Saranholi, Bruno H. ^{[1, 4]} ; Galetti Jr, Pedro M. Jr

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Genet & Evolucao, Sao Carlos, SP - Brazil

^[2] Inst Tecnol Vale, BR-66055090 Belem, Para - Brazil

^[3] Univ Llanos, Fac Ciencias Basicas & Ingn, Villavicencio - Colombia

^[4] Imperial Coll London, Dept Life Sci, London - England

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR ECOLOGY RESOURCES; NOV 2021.
• Citações Web of Science: 0

Resumo

Environmental DNA (eDNA) and invertebrate-derived DNA (iDNA) have been increasingly recognized as powerful tools for biodiversity assessment and conservation management. However, eDNA/iDNA efficiency for vertebrate diversity assessment remains uncertain, and comparisons to conventional methods are still rare. Through a meta-analysis of previously published vertebrate diversity surveys, we compared the efficiency of eDNA/iDNA against conventional methods across several types of samplers, vertebrate groups, and locations (tropical vs. temperate zones). We also assess eDNA/iDNA efficiency to estimate relative abundance or biomass over different molecular methods (qPCR and metabarcoding) and type of experiment (in the laboratory or in the field). We showed that for water sampler, fish as a target species, and studies achieved in temperate zones, eDNA presents lower risk of not detecting a species or a site with a target species than conventional methods. These results show that eDNA is an efficient tool to assess fish diversity. Moreover, eDNA data presents positive correlation with fish abundance or biomass. However, such correlation was higher in laboratory experiments than in the field. For the other samplers, vertebrate groups, and in tropical zones we were not able to draw general conclusion, highlighting the urgency of conducting more comparative studies. (AU)

• Processo FAPESP: 19/26436-6 - Reavaliação das espécies de mamíferos que se acredita estar localmente extintos no Centro de Endemismo Pernambuco (CEP), a partir de DNA de amostras mistas e metabarcoding
• Beneficiário: Carolina da Silva Carvalho
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado