| Texto completo | |
| Autor(es): |
Santos, Barbara
[1]
;
Zeng, Rui
[2]
;
Jorge, Sasa F.
[1]
;
Ferreira-Junior, Jose Ribamar
[3]
;
Barrientos, Antoni
[2]
;
Barros, Mario H.
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Dept Microbiol, Sao Paulo - Brazil
[2] Univ Miami, Miller Sch Med, Dept Neurol, Miami, FL 33136 - USA
[3] Univ Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo - Brazil
Número total de Afiliações: 3
|
| Tipo de documento: | Artigo Científico |
| Fonte: | YEAST; NOV 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
In Saccharomyces cerevisiae, mitoribosomes are composed of a 54S large subunit (mtLSU) and a 37S small subunit (mtSSU). The two subunits altogether contain 73 mitoribosome proteins (MRPs) and two ribosomal RNAs (rRNAs). Although mitoribosomes preserve some similarities with their bacterial counterparts, they have significantly diverged by acquiring new proteins, protein extensions, and new RNA segments, adapting the mitoribosome to the synthesis of highly hydrophobic membrane proteins. In this study, we investigated the functional relevance of mitochondria-specific protein extensions at the C-terminus (C) or N-terminus (N) present in 19 proteins of the mtLSU. The studied mitochondria-specific extensions consist of long tails and loops extending from globular domains that mainly interact with mitochondria-specific proteins and 21S rRNA moieties extensions. The expression of variants devoid of extensions in uL4 (C), uL5 (N), uL13 (N), uL13 (C), uL16 (C), bL17 (N), bL17 (C), bL21 (24), uL22 (N), uL23 (N), uL23 (C), uL24 (C), bL27 (C), bL28 (N), bL28 (C), uL29 (N), uL29 (C), uL30 (C), bL31 (C), and bL32 (C) did not rescue the mitochondrial protein synthesis capacities and respiratory growth of the respective null mutants. On the contrary, the truncated form of the mitoribosome exit tunnel protein uL24 (N) yields a partially functional mitoribosome. Also, the removal of mitochondria-specific sequences from uL1 (N), uL3 (N), uL16 (N), bL9 (N), bL19 (C), uL29 (C), and bL31 (N) did not affect the mitoribosome function and respiratory growth. The collection of mutants described here provides new means to study and evaluate defective assembly modules in the mitoribosome biogenesis process. (AU) | |
| Processo FAPESP: | 13/07937-8 - Redoxoma |
| Beneficiário: | Ohara Augusto |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |
| Processo FAPESP: | 20/05812-7 - Eventos regulatórios na biogênese do mitorribossomo e dos complexos respiratórios |
| Beneficiário: | Mario Henrique de Barros |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 18/03253-0 - Formação do túnel de saída do mitoribossomo e o seu papel na tradução mitocondrial |
| Beneficiário: | Barbara Santos de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Iniciação Científica |
| Processo FAPESP: | 17/23921-5 - Estudo de fatores envolvidos na tradução mitocondrial |
| Beneficiário: | Mario Henrique de Barros |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |