Effect of the protein structure and heme iron coordination sphere on the long-range electron transfer from hematite and zinc oxide nanostructures to cytochrome c

Autor(es)

Menezes, Lucivaldo R. ^[1] ; Araujo-Chaves, Juliana C. ^[1] ; Lopes, David M. ^[1] ; Bronzato, Julia D. ^[1] ; Sombrio, Guilherme ^[1] ; Criado, Denise ^[1] ; Zuniga, Alejandro ^[2] ; Lanfredi, Alexandre J. C. ^[2] ; Souza, Jose A. ^[1] ; Nantes-Cardoso, Iseli L. ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC, Ctr Nat Sci & Humanities, BR-09210580 Santo Andre, SP - Brazil

^[2] Fed Univ ABC, Ctr Engn & Appl Social Sci, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Nanotechnology; v. 17, n. 1, SI, p. 42-56, 2020.
• Citações Web of Science: 2

Resumo

Cytochrome c has gained increasing interest in non-biological areas because of its electronic and magnetic properties that are applicable in spintronics and energy. The chiral helicoidal structure of cytochrome c gives to this protein the property of chiral-induced spin selectivity (CISS) applicable to spintronics and energy. In a previous study, it was demonstrated that Fe2O3, structured as layers of nanowires (NWs) and nanoflakes (NFs), contrary to ZnONWs, can efficiently reduce cytochrome c heme when submitted to illumination with a sunlight simulator. The present study investigated the influence of alpha-helix content and heme iron axial ligands on the capacity of cytochrome c to accept electrons from Fe(2)O(3)NWs (NFs) pristine and decorated with gold nanoparticles. Microperoxidase-11 (MP-11), a heme-undecapeptide produced by cytochrome c digestion was irradiated in the presence of Fe(2)O(3)NFs. Efficient photoreduction was observed only for MP-11 with hexacoordinated heme iron. The results demonstrated that the heme coordination sphere rather than the alpha-helix content is crucial for the efficient long-range electron transfer from hematite to heme iron. Also, ZnO grown as nanoflowers (NFws) at room temperature was tested as a photoreduction agent, and like ZnONWs was also an inefficient photoreduction agent for cytochrome c. (AU)

• Processo FAPESP: 17/02317-2 - Interfaces em materiais: propriedades eletrônicas, magnéticas, estruturais e de transporte
• Beneficiário: Adalberto Fazzio
• Modalidade de apoio: Auxílio à Pesquisa - Temático