Effects of Qualea grandiflora Extract on the Expression of MMP-14 and HIF-1 alpha in Cultured Fibroblasts and Preosteoblasts

Autor(es)

Neubern de Oliveira, Gabriela Silva ^[1] ; Tokuhara, Cintia Kazuko ^[1] ; de Oliveira, Flavia Amadeu ^[1] ; Zardin Graeff, Marcia Sirlene ^[1] ; Khan, Zohaib Nisar ^[1] ; Saldanha, Luiz Leonardo ^[2] ; Dokkedal, Anne Ligia ^[2] ; de Oliveira, Rodrigo Cardoso ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP - Brazil

^[2] Sao Paulo State Univ, Fac Sci, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Archives of Biology and Technology; v. 64, 2021.
• Citações Web of Science: 0

Resumo

Background: Qualea grandiflora (QG) (Vochysiaceae), also known as ``pau-ferro{''}, ``pau-terra{''} or ``pau-de-tucano{''}, is a very common deciduous tree in the Brazilian Cerrado used in traditional medicine to treat inflammations, ulcers, diarrhea, and infections. There are reports in the scientific literature that demonstrate the medicinal effects of the bark and leaf of the QG. However, studies involving this plant are rather imited. Aim of the study: To perform the phytochemical analysis of the QG hydroalcoholic extract (HAE) of leaves, and to investigate it effects on fibroblast and preosteoblasts. Methods: Phytochemical analysis was done by HPLC-DAD. Murine NIH/3T3 fibroblasts and MC3T3-E1 preosteoblasts cell lines (ATCC) were used for the experiments. Cell viability was assessed by the MTT colorimetric assay and the expression of MMP-14 and HIF-1 alpha by immunofluorescence. Results and conclusion: The following compounds were identified by HPLC-DAD, such as quinic acid, ethyl galate, ellagic acid derivatives as O-methylellagic acid O-galloyl, O-methylellagic acid O-deoxyhexoside, galloyl derivatives, flavonol glycoside as kaempferol-O-deoxyhexoside, quercetin-O-deoxyhexoside, myricetin-O-deoxyhexoside and the pentacyclic triterpene arjunglucoside. Cell viability results demonstrated no cytotoxic effects in the studied concentrations. We found in QG HAE some compounds with therapeutic properties that can increase the expression of MMP-14 and HIF-1 alpha, in fibroblasts and preosteoblasts. These data suggest that QG HAE has an action on these two molecules widely involved in physiological conditions, such as collagen remodeling, bone development and growth and pathological processes as HIF signaling in cancer metastasis. (AU)

• Processo FAPESP: 14/05234-2 - Estudo do extrato hidroalcoólico de folhas de Qualea grandiflora: avaliação fitoquímica e efeitos na expressão de proteínas envolvidas em processos inflamatórios
• Beneficiário: Rodrigo Cardoso de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/20656-0 - Avaliação do efeito da Qualea grandiflora Mart na modulação de HIF-1 alpha e MMP-14 em linhagens celulares de fibroblastos e pré-osteoblastos
• Beneficiário: Gabriela Silva Neubern de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Mestrado