| Texto completo | |
| Autor(es): |
Alonso, Diego Peres
[1, 2, 3]
;
Niz Alvarez, Marcus Vinicius
[1, 2]
;
Martins Ribolla, Paulo Eduardo
[1, 2]
;
Conn, Jan E.
[4, 5]
;
Porangaba de Oliveira, Tatiane Marques
[3]
;
Mureb Sallum, Maria Anice
[3]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Sao Paulo State Univ UNESP, Biotechnol Inst, BR-18618689 Botucatu, SP - Brazil
[2] Sao Paulo State Univ UNESP, Biosci Inst, BR-18618689 Botucatu, SP - Brazil
[3] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-05508060 Sao Paulo - Brazil
[4] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12159 - USA
[5] SUNY Albany, Dept Biomed Sci, Sch Publ Hlth, Albany, NY 12222 - USA
Número total de Afiliações: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENES; v. 12, n. 11 NOV 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
Mosquito susceptibility to Plasmodium spp. infection is of paramount importance for malaria occurrence and sustainable transmission. Therefore, understanding the genetic features underlying the mechanisms of susceptibility traits is pivotal to assessing malaria transmission dynamics in endemic areas. The aim of this study was to investigate the susceptibility of Nyssorhynchus darlingi-the dominant malaria vector in Brazil-to Plasmodium spp. using a reduced representation genome-sequencing protocol. The investigation was performed using a genome-wide association study (GWAS) to identify mosquito genes that are predicted to modulate the susceptibility of natural populations of the mosquito to Plasmodium infection. After applying the sequence alignment protocol, we generated the variant panel and filtered variants; leading to the detection of 202,837 SNPs in all specimens analyzed. The resulting panel was used to perform GWAS by comparing the pool of SNP variants present in Ny. darlingi infected with Plasmodium spp. with the pool obtained in field-collected mosquitoes with no evidence of infection by the parasite (all mosquitoes were tested separately using RT-PCR). The GWAS results for infection status showed two statistically significant variants adjacent to important genes that can be associated with susceptibility to Plasmodium infection: Cytochrome P450 (cyp450) and chitinase. This study provides relevant knowledge on malaria transmission dynamics by using a genomic approach to identify mosquito genes associated with susceptibility to Plasmodium infection in Ny. darlingi in western Amazonian Brazil. (AU) | |
| Processo FAPESP: | 14/26229-7 - Genômica de paisagens em gradientes latitudinais e ecologia de Anopheles darlingi |
| Beneficiário: | Maria Anice Mureb Sallum |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |