Atlantic Forest's and Caatinga's semiarid soils and their potential as a source for halothermotolerant actinomycetes and proteolytic enzymes

Autor(es)

Silveira, Marghuel A. Vieira ^[1] ; Batista dos Santos, Saara M. ^[2] ; Okamoto, Debora Noma ^[3] ; de Melo, Itamar Soares ^[4] ; Juliano, Maria A. ^[2] ; Ribeiro Chagas, Jair ^[2] ; Vasconcellos, Suzan P. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Biophys, Lab Biochem, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Inst Pharmacol & Mol Biol, Dept Biophys, Lab Biochem & Enzymol, Escola Paulista Med, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo, Dept Pharmaceut Sci, Lab Hlth & Environm Sci, BR-09913030 Diadema - Brazil

^[4] Brazilian Agr Res Corp, EMBRAPA Environm, Lab Environm Microbiol, Jaguariuna - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ENVIRONMENTAL TECHNOLOGY; DEC 2021.
• Citações Web of Science: 2

Resumo

Actinomycetes are versatile about their metabolism, displaying high capacity to produce bioactive metabolites. Enzymes from actinomycetes represent new opportunities for industrial applications. However, proteases from actinomycetes are poorly described by literature. Thereby, to verify proteolytic potential of actinomycetes, the present study aimed the investigation of bacterial isolates from Caatinga and Atlantic Forest rhizosphere. Fluorescence resonance energy transfer (FRET) peptide libraries were adopted for the evaluations, since they are faster and more qualitative methods, if compared with others described by most reports. A total of 52 microorganisms were inoculated in different culture media (PMB, potato dextrose agar, brain heart infusion agar, Starch Casein Agar and Reasoner's 2A agar), temperatures (12, 20, 30, 37, 45 and 60 degrees C), and saline conditions (0-4 M NaCl), during 7 days. The actinomycetes named as AC 01, 02 and 52 were selected and showed enzymatic abilities under the peptide probes Abz-KLRSSKQ-EDDnp and Abz-KLYSSKQ-EDDnp, achieving enhanced performance at 30 degrees C. Biochemical parameters were established, showing a predominance of alkaline proteases with activity under saline conditions. Secreted proteases hydrolysed preferentially polar uncharged residues (Y and N) and positively charged groups (R). Phenylmethylsulfonyl fluoride and ethylenediaminetetraacetic acid inhibited the proteins, a characteristic of serine (AC 01 e 02) and metalloproteases (AC 52). All selected strains belonged to Streptomyces genera. In summary, actinomycete strains with halophilic proteolytic abilities were selected, which improve possibilities for their use in detergent formulations, food processing, waste management and industrial bioconversion. It is important to highlight that this is the first report using FRET libraries for proteolytic screening from Caatinga and Atlantic Forest actinobacteria. (AU)

• Processo FAPESP: 10/51992-5 - Investigação do potencial biotecnológico de microrganismos isolados do processo de compostagem de resíduos do Zoológico de São Paulo
• Beneficiário: Suzan Pantaroto de Vasconcellos
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/23685-7 - Estudo da biotransformação de dímeros de Fenil-propanóides por actinomicetos da caatinga
• Beneficiário: Suzan Pantaroto de Vasconcellos
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular