Correlating biological activity to thermo-structural analysis of the interaction of CTX with synthetic models of macrophage membranes

Autor(es)

de Araujo Pimenta, Luciana ^{[1, 2]} ; Duarte, Evandro L. ^[3] ; Muniz, Gabriel S. Vignoli ^[3] ; Pasqualoto, Kerly Fernanda Mesquita ^[4] ; de Mattos Fontes, Marcos Roberto ^[5] ; Lamy, M. Teresa ^[3] ; Sampaio, Sandra Coccuzzo ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Inst Butantan, Pathophysiol Lab, Av Vital Brazil 1500, BR-05503900 Butanta, SP - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Av Lineu Prestes 1524, BR-05508900 Butanta, SP - Brazil

^[3] Univ Sao Paulo, Inst Phys, R Matao 1371, BR-05508090 Butanta, SP - Brazil

^[4] Alchemy Inovacao Pesquisa & Dev Ltda, Technol Based Business Incubator Sao Paulo IPEN U, Av Lineu Prestes 2242 Bldg D, BR-05508000 Butanta, SP - Brazil

^[5] Univ Estadual Paulista, Inst Biosci, Dept Biophys & Pharmacol, Prof Dr Antonio Celso Wagner Zanin 250, BR-18618689 Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 11, n. 1 DEC 9 2021.
• Citações Web of Science: 0

Resumo

The important pharmacological actions of Crotoxin (CTX) on macrophages, the main toxin in the venom of Crotalus durissus terrificus, and its important participation in the control of different pathophysiological processes, have been demonstrated. The biological activities performed by macrophages are related to signaling mediated by receptors expressed on the membrane surface of these cells or opening and closing of ion channels, generation of membrane curvature and pore formation. In the present work, the interaction of the CTX complex with the cell membrane of macrophages is studied, both using biological cells and synthetic lipid membranes to monitor structural alterations induced by the protein. Here we show that CTX can penetrate THP-1 cells and induce pores only in anionic lipid model membranes, suggesting that a possible access pathway for CTX to the cell is via lipids with anionic polar heads. Considering that the selectivity of the lipid composition varies in different tissues and organs of the human body, the thermostructural studies presented here are extremely important to open new investigations on the biological activities of CTX in different biological systems. (AU)

• Processo FAPESP: 17/11161-6 - Interação Crotoxina e Macrófagos: Estudo da Participação de Receptor Peptídeo Formil e de Domínios Lipídicos nos Mecanismos Envolvidos no Processo de Reconhecimento Molecular Toxina-Membrana
• Beneficiário: Sandra Coccuzzo Sampaio Vessoni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/50983-3 - INCT 2014: fluidos complexos
• Beneficiário: Antonio Martins Figueiredo Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático