olecular Analysis Reveals a High Diversity of Anopheline Mosquitoes in Yanomami Lands and the Pantanal Region of Brazi

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Silva-do-Nascimento, Teresa Fernandes ^[1] ; Sanchez-Ribas, Jordi ^{[1, 2, 3]} ; Oliveira, Tatiane M. P. ^[4] ; Bourke, Brian Patrick ^{[5, 6, 7]} ; Oliveira-Ferreira, Joseli ^[1] ; Rosa-Freitas, Maria Goreti ^{[1, 8]} ; Lourenco-de-Oliveira, Ricardo ^[1] ; Marinho-e-Silva, Mariana ^{[1, 9]} ; Neves, Maycon Sebastiao Alberto Santos ^[1] ; Conn, Jan E. ^{[10, 11]} ; Sallum, Maria Anice Mureb ^[4]

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^[1] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Mosquitos Transmissores Hematozoarios, BR-21040360 Rio De Janeiro - Brazil

^[2] Fundacao Oswaldo Cruz, Inst Oswaldo Cruz, Lab Imunoparasitol, BR-21040360 Rio De Janeiro - Brazil

^[3] Distrito Sanitario Especial Indigena Yanomami, BR-69301080 Roraima - Brazil

^[4] Univ Sao Paulo, Fac Saude Publ, Dept Epidemiol, BR-01246904 Sao Paulo - Brazil

^[5] Walter Reed Army Inst Res, 503 Robert Grant Ave, Silver Spring, MD 20910 - USA

^[6] Smithsonian Inst, Museum Support Ctr MRC 534, Walter Reed Biosystemat Unit, 4210 Silver Hill Rd, Suitland, MD 20746 - USA

^[7] Natl Museum Nat Hist, Smithsonian Inst, Dept Entomol, 10th St NE & Constitut Ave NE, Washington, DC 20002 - USA

^[8] Geniac Ltd, BR-01031902 Sao Paulo - Brazil

^[9] Inst Nacl Propriedade Ind, BR-20090910 Rio De Janeiro - Brazil

^[10] SUNY Albany, Sch Publ Hlth, Dept Biomed Sci, Albany, NY 12222 - USA

^[11] New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12159 - USA

• Tipo de documento: Artigo Científico
• Fonte: GENES; v. 12, n. 12 DEC 2021.
• Citações Web of Science: 0

Resumo

Identifying the species of the subfamily Anophelinae that are Plasmodium vectors is important to vector and malaria control. Despite the increase in cases, vector mosquitoes remain poorly known in Brazilian indigenous communities. This study explores Anophelinae mosquito diversity in the following areas: (1) a Yanomami reserve in the northwestern Amazon Brazil biome and (2) the Pantanal biome in southwestern Brazil. This is carried out by analyzing cytochrome c oxidase (COI) gene data using Refined Single Linkage (RESL), Assemble Species by Automatic Partitioning (ASAP), and tree-based multi-rate Poisson tree processes (mPTP) as species delimitation approaches. A total of 216 specimens collected from the Yanomami and Pantanal regions were sequenced and combined with 547 reference sequences for species delimitation analyses. The mPTP analysis for all sequences resulted in the delimitation of 45 species groups, while the ASAP analysis provided the partition of 48 groups. RESL analysis resulted in 63 operational taxonomic units (OTUs). This study expands our scant knowledge of anopheline species in the Yanomami and Pantanal regions. At least 18 species of Anophelinae mosquitoes were found in these study areas. Additional studies are now required to determine the species that transmit Plasmodium spp. in these regions. (AU)

• Processo FAPESP: 14/26229-7 - Genômica de paisagens em gradientes latitudinais e ecologia de Anopheles darlingi
• Beneficiário: Maria Anice Mureb Sallum
• Modalidade de apoio: Auxílio à Pesquisa - Temático