pplication of Electron Spin Resonance Spectroscopy in Bone Regeneration Studie

Autor(es)

Blasioli, Gisele Janaina ^[1] ; Pereira Silva, Lucas Henrique ^{[2, 3]} ; Holgado, Leandro Andrade ^[1] ; Telles de Sousa, Tatiana Peixoto ^[1] ; Catanzaro Guimaraes, Sergio Augusto ^[4] ; Baffa, Oswaldo ^[5] ; Kinoshita, Angela ^[3]

Afiliação do(s) autor(es)

^[1] Unisagrad, Ctr Univ Sagrad Coracao, BR-17011160 Bauru, SP - Brazil

^[2] Inst Fed Sao Paulo, Sao Paulo, SP - Brazil

^[3] Univ Oeste Paulista, UNOESTE, BR-19067175 Presidente Prudente, SP - Brazil

^[4] Univ Sao Paulo, FOB Fac Odontol Bauru, BR-1701290 Bauru, SP - Brazil

^[5] Univ Sao Paulo, Dept Fis, FFCLRP, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Physics; v. 52, n. 2 APR 2022.
• Citações Web of Science: 0

Resumo

Electron spin resonance (ESR) is a spectroscopic technique that detects unpaired electrons, such as the ones present in free radicals. When a calcified tissue is irradiated, free radicals are generated, like the CO2-. This paper presents a preliminary study on the application of this spectroscopic technique for monitoring bone mineralization processes during the bone repair in animal model, induced by polyurethane in two forms, as an occlusive membrane and as a bone grafting. In the first experiment, the ESR results and microscopic analysis demonstrate centripetal bone growth and advanced stage of bone regeneration in the treated group. In the second experiment, the ESR spectrum of collected bone pieces presents the superposition of the spectrum of the biomaterial and the radical CO2-. The separation of the components was made using spectral simulation, and the percentage of mature bone tissue in the defect region was determined through the comparison with the spectrum of the native bone. In both experiments, ESR provided results consistent with microscopic analysis, demonstrating the feasibility of the technique for monitoring bone regeneration. (AU)

• Processo FAPESP: 13/07699-0 - Centro de Pesquisa, Inovação e Difusão em Neuromatemática - NeuroMat
• Beneficiário: Oswaldo Baffa Filho
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 07/06720-4 - Avanços em dosimetria, datação arqueológica e caracterização de biomateriais por ressonância de spin eletrônico
• Beneficiário: Oswaldo Baffa Filho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 03/09505-6 - Uso da Ressonância do Spin Eletrônico (RSE) no estudo da regeneração óssea guiada por membrana de resina de poliuretano e membrana de politetrafluoretileno
• Beneficiário: Angela Mitie Otta Kinoshita
• Modalidade de apoio: Auxílio à Pesquisa - Regular