pplication of Electron Spin Resonance Spectroscopy in Bone Regeneration Studie

Author(s)

Blasioli, Gisele Janaina ^[1] ; Pereira Silva, Lucas Henrique ^{[2, 3]} ; Holgado, Leandro Andrade ^[1] ; Telles de Sousa, Tatiana Peixoto ^[1] ; Catanzaro Guimaraes, Sergio Augusto ^[4] ; Baffa, Oswaldo ^[5] ; Kinoshita, Angela ^[3]

Affiliation

^[1] Unisagrad, Ctr Univ Sagrad Coracao, BR-17011160 Bauru, SP - Brazil

^[2] Inst Fed Sao Paulo, Sao Paulo, SP - Brazil

^[3] Univ Oeste Paulista, UNOESTE, BR-19067175 Presidente Prudente, SP - Brazil

^[4] Univ Sao Paulo, FOB Fac Odontol Bauru, BR-1701290 Bauru, SP - Brazil

^[5] Univ Sao Paulo, Dept Fis, FFCLRP, BR-14049900 Ribeirao Preto, SP - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Physics; v. 52, n. 2 APR 2022.
• Web of Science Citations: 0

Abstract

Electron spin resonance (ESR) is a spectroscopic technique that detects unpaired electrons, such as the ones present in free radicals. When a calcified tissue is irradiated, free radicals are generated, like the CO2-. This paper presents a preliminary study on the application of this spectroscopic technique for monitoring bone mineralization processes during the bone repair in animal model, induced by polyurethane in two forms, as an occlusive membrane and as a bone grafting. In the first experiment, the ESR results and microscopic analysis demonstrate centripetal bone growth and advanced stage of bone regeneration in the treated group. In the second experiment, the ESR spectrum of collected bone pieces presents the superposition of the spectrum of the biomaterial and the radical CO2-. The separation of the components was made using spectral simulation, and the percentage of mature bone tissue in the defect region was determined through the comparison with the spectrum of the native bone. In both experiments, ESR provided results consistent with microscopic analysis, demonstrating the feasibility of the technique for monitoring bone regeneration. (AU)

• FAPESP's process: 13/07699-0 - Research, Innovation and Dissemination Center for Neuromathematics - NeuroMat
• Grantee: Oswaldo Baffa Filho
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 07/06720-4 - Advances in electron spin resonance dosimetry, archeological dating and biomaterials characterization
• Grantee: Oswaldo Baffa Filho
• Support Opportunities: Regular Research Grants