sing crystallography tools to improve vaccine formulation

Autor(es):Mostrar menos -

de Abreu Fantini, Marcia Carvalho ^[1] ; Pinto Oliveira, Cristiano Luis ^[1] ; de Souza Lopes, Jose Luiz ^[1] ; Martins, Tereza da Silva ^[2] ; Akamatsu, Milena Apetito ^[3] ; Trezena, Aryene Goes ^[4] ; Tino-De-Franco, Milene ^[4] ; Botosso, Viviane Fongaro ^[5] ; Brazil Esteves Sant'Anna, Osvaldo Augusto ^[6] ; Kardjilov, Nikolay ^[7] ; Rasmussen, Martin Kjaerulf ^[8] ; Bordallo, Heloisa Nunes ^[8]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Phys Inst, Rua Matao 1371, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Fed Sao Paulo, Chem Dept, Rua Sao Nicolau 210, 2o Andar, BR-09913030 Diadema, SP - Brazil

^[3] Butantan Inst, Bioind Ctr, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[4] Butantan Inst, Immunogenet Lab, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[5] Butantan Inst, Virol Lab, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[6] Butantan Inst, Immunochem Lab, Ave Vital Brazil 1500, BR-05503900 Sao Paulo, SP - Brazil

^[7] Helmholtz Zentrum Berlin, HZB Mat & Energie, Hahn Meitner Pl 1, D-14109 Berlin - Germany

^[8] Univ Copenhagen, Niels Bohr Inst, Univ Pk 5, DK-2100 Copenhagen - Denmark

• Tipo de documento: Artigo de Revisão
• Fonte: IUCRJ; v. 9, n. 1, p. 11+, JAN 2022.
• Citações Web of Science: 0

Resumo

This article summarizes developments attained in oral vaccine formulations based on the encapsulation of antigen proteins inside porous silica matrices. These vaccine vehicles show great efficacy in protecting the proteins from the harsh acidic stomach medium, allowing the Peyer's patches in the small intestine to be reached and consequently enhancing immunity. Focusing on the pioneering research conducted at the Butantan Institute in Brazil, the optimization of the antigen encapsulation yield is reported, as well as their distribution inside the meso- and macroporous network of the porous silica. As the development of vaccines requires proper inclusion of antigens in the antibody cells, X-ray crystallography is one of the most commonly used techniques to unveil the structure of antibody-combining sites with protein antigens. Thus structural characterization and modelling of pure antigen structures, showing different dimensions, as well as their complexes, such as silica with encapsulated hepatitis B virus-like particles and diphtheria anatoxin, were performed using small-angle X-ray scattering, X-ray absorption spectroscopy, X-ray phase contrast tomography, and neutron and X-ray imaging. By combining crystallography with dynamic light scattering and transmission electron microscopy, a clearer picture of the proposed vaccine complexes is shown. Additionally, the stability of the immunogenic complex at different pH values and temperatures was checked and the efficacy of the proposed oral immunogenic complex was demonstrated. The latter was obtained by comparing the antibodies in mice with variable high and low antibody responses. (AU)

• Processo FAPESP: 17/17844-8 - Sílica nanoestruturada como veículo protetor de vacinas e biomoléculas
• Beneficiário: Osvaldo Augusto Brazil Esteves Sant'Anna
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/19546-7 - Mecanismos moleculares da ligação, inserção e orientação de peptídeos antimicrobianos em modelos de membrana
• Beneficiário: Jose Luiz de Souza Lopes
• Modalidade de apoio: Auxílio à Pesquisa - Regular