iological and Inflammatory Effects of Antigen 5 from Polybia paulista (Hymenoptera, Vespidae) Venom in Mouse Intraperitoneal Macrophage

Autor(es)

Bazon, Murilo Luiz ^[1] ; Fernandes, Luis Gustavo Romani ^[2] ; Assugeni, Isabela Oliveira Sandrini ^[1] ; Pinto, Lucas Machado ^[1] ; Simioni, Patricia Ucelli ^[3] ; Zollner, Ricardo de Lima ^{[2, 4]} ; Braga, Marcia Regina Brochetto ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista Campus Rio Claro UNESP RC, Inst Biol IB, Lab Biol Mol Artropodes LBMA, Ave 24-A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Fac Ciencias Med FCM, Dept Clin Med, Lab Imunol Translac LIT, Rua Tessalia Vieira Camargo 126, BR-13083887 Campinas, SP - Brazil

^[3] Fac Amer FAM, Dept Biomed, Ave Joaquim Boer 733, BR-13477360 Americana, SP - Brazil

^[4] Univ Anhembi Morumbi, Fac Med, Av Rio Pedras 1601, BR-13425380 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: TOXINS; v. 13, n. 12 DEC 2021.
• Citações Web of Science: 0

Resumo

The social wasp Polybia paulista (Hymenoptera, Vespidae) is highly aggressive, being responsible for many medical occurrences. One of the most allergenic components of this venom is Antigen 5 (Poly p 5). The possible modulation of the in vitro immune response induced by antigen 5 from P. paulista venom, expressed recombinantly (rPoly p 5), on BALB/c mice peritoneal macrophages, activated or not with LPS, was assessed. Here, we analyzed cell viability changes, expression of the phosphorylated form of p65 NF-kappa B subunit, nitric oxide (NO), proinflammatory cytokines production, and co-stimulatory molecules (CD80, CD86). The results suggest that rPoly p 5 does not affect NO production nor the expression of co-stimulatory molecules in mouse peritoneal macrophages. On the other hand, rPoly p 5 induced an increase in IL-1 beta production in non-activated macrophages and a reduction in the production of TNF-alpha and MCP-1 cytokines in activated macrophages. rPoly p 5 decreased the in vitro production of the phosphorylated p65 NF-kappa B subunit in non-activated macrophages. These findings suggest an essential role of this allergen in the polarization of functional M2 macrophage phenotypes, when analyzed in previously activated macrophages. Further investigations, mainly in in vivo studies, should be conducted to elucidate Polybia paulista Ag5 biological role in the macrophage functional profile modulation. (AU)

• Processo FAPESP: 17/07988-2 - Avaliação biológica e inflamatória dos efeitos do antígeno 5 do veneno de Polybia paulista (Hymenoptera, Vespidae) na forma recombinante, sobre células de linhagem macrofágica
• Beneficiário: Murilo Luiz Bazon
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/18422-0 - Avaliação dos efeitos do Antígeno 5 do veneno de Polybia paulista (Hymenoptera,Vespidae) na forma recombinante sobre a produção de mediadores inflamatórios e imunológicos in vitro para o desenvolvimento de kit diagnóstico
• Beneficiário: Márcia Regina Brochetto Braga
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 19/02298-3 - Caracterização do potencial imunológico do antígeno recombinante 5 do veneno de Polybia paulista
• Beneficiário: Murilo Luiz Bazon
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado