-Glycoprotein and Androgen Receptor Expression Reveals Independence of Canine Prostate Cancer from Androgen Hormone Stimulatio

Autor(es)

Cavalca, Alexandre Matheus Baesso ^[1] ; Brandi, Andressa ^[1] ; Fonseca-Alves, Ricardo Henrique ^[2] ; Laufer-Amorim, Renee ^[3] ; Fonseca-Alves, Carlos Eduardo ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Surg & Anesthesiol, BR-18618687 Botucatu, SP - Brazil

^[2] Fed Univ Goias UFG, Sch Elect Mech & Comp Engn, Dept Elect Engn, BR-74690900 Goiania, Go - Brazil

^[3] Sao Paulo State Univ UNESP, Sch Vet Med & Anim Sci, Dept Vet Clin, BR-18618687 Botucatu, SP - Brazil

^[4] Paulista Univ UNIP, Inst Hlth Sci, BR-17048290 Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 3 FEB 2022.
• Citações Web of Science: 0

Resumo

Canine prostate cancer (PC) is an aggressive disease, and dogs can be considered comparative models for human PC. In recent years, canine PC has been shown to resemble human castrate-resistant prostate cancer. The influx and efflux of testosterone in prostatic luminal cells are regulated by P-glycoprotein (P-gp). Therefore, human PC generally lacks P-gp expression and maintains the expression of androgen receptors (ARs). However, this co-expression has not previously been investigated in dogs. Therefore, this study aimed to evaluate AR and P-gp co-expression to elucidate these protein patterns in canine prostate samples. We identified AR/P-gp double immunofluorescence co-expression of both proteins in normal luminal cells. However, in canine PC, cells lack AR expression and exhibit increased P-gp expression. These results were confirmed by gene expression analyses. Overall, our results strongly suggest that normal canine prostate testosterone influx may be regulated by P-gp expression, and that during progression to PC, prostatic cells lack AR expression and P-gp overexpress. P-gp expression in canine PC may be related to a phenotype of multiple drug resistance. (AU)

• Processo FAPESP: 15/25400-7 - Estudo para caracterização in vitro e molecular de células prostáticas caninas e avaliação da resposta antitumoral a drogas alvo dirigidas
• Beneficiário: Carlos Eduardo Fonseca Alves
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado