apping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coatin

Autor(es)

Faverani, Leonardo P. ^[1] ; Silva, William P. P. ^[1] ; de Sousa, Cecilia Alves ^[2] ; Freitas, Gileade ; Bassi, Ana Paula F. ^[1] ; Shibli, Jamil A. ^[3] ; Barao, Valentim A. R. ^[4] ; Rosa, Adalberto L. ^[5] ; Sukotjo, Cortino ^[6] ; Assuncao, Wirley G. ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dept Diag & Surg, Div Oral & Maxillofacial Surg & Implantol, Sch Dent, BR-16015050 Aracatuba - Brazil

^[2] Sao Paulo State Univ UNESP, Dent Prosthesis Dept Dent Mat & Prosthodont, Sch Dent, BR-16015050 Aracatuba - Brazil

^[3] Univ Guarulhos UnG, Dent Res Div, Dept Periodontol & Oral Implantol, BR-07023070 Guarulhos - Brazil

^[4] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, BR-13414903 Piracicaba - Brazil

^[5] Univ Sao Paulo, Bone Res Lab, Sch Dent Ribeirao Preto, BR-14040904 Ribeirao Preto - Brazil

^[6] Univ Illinois, Dept Restorat Dent, Coll Dent, Chicago, IL 60612 - USA

• Tipo de documento: Artigo Científico
• Fonte: MATERIALS; v. 15, n. 3 FEB 2022.
• Citações Web of Science: 0

Resumo

Chemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats' femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p < 0.05) and from 7 to 10 days (p < 0.05) was noted for PEO and SLA surfaces. Gene expression for OSX, ALP, BSP, and OPN showed a statistical significance (p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations. (AU)

• Processo FAPESP: 16/20297-6 - A influência da texturização de superfície do Titânio comercialmente puro (Ticp) com incorporação de Ca e P produzida pela oxidação por plasma eletrolítico em ossos de baixa densidade: Um estudo in vitro, ex-vivo e in vivo
• Beneficiário: Leonardo Perez Faverani
• Modalidade de apoio: Auxílio à Pesquisa - Regular