LA/Hydroxyapatite scaffolds exhibit in vitro immunological inertness and promote robust osteogenic differentiation of human mesenchymal stem cells without osteogenic stimul

Autor(es)

Bernardo, Marcela P. ^{[1, 2]} ; da Silva, Bruna C. R. ^[2] ; Hamouda, I, Ahmed E. ; de Toledo, Marcelo A. S. ^[3] ; Schalla, Carmen ^[4] ; Rutten, Stephan ^[5] ; Goetzke, Roman ^[6] ; Mattoso, Luiz H. C. ^[2] ; Zenke, Martin ^[4] ; Sechi, Antonio ^[4]

Afiliação do(s) autor(es)

^[1] Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany

^[2] Natl Nanotechnol Lab Agribusiness, Brazilian Agr Res Corp, Embrapa Instrumentat, Rua 15 Novembro, BR-1452 Sao Carlos, SP - Brazil

^[3] Rhein Westfal TH Aachen, Dept Hematol Oncol Hemostaseol & Stem Cell Transp, D-52074 Aachen - Germany

^[4] Hamouda, Ahmed E., I, Rhein Westfal TH Aachen, Inst Biomed Engn, Dept Cell Biol, D-52074 Aachen - Germany

^[5] Rhein Westfal TH Aachen, Electron Microscopy Facil, D-52074 Aachen - Germany

^[6] Rhein Westfal TH Aachen, Helmholtz Inst Biomed Engn, Stem Cell Biol & Cellular Engn, D-52074 Aachen - Germany

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 12, n. 1 FEB 11 2022.
• Citações Web of Science: 0

Resumo

Bone defects stand out as one of the greatest challenges of reconstructive surgery. Fused deposition modelling (FDM) allows for the printing of 3D scaffolds tailored to the morphology and size of bone damage in a patient-specific and high-precision manner. However, FDM still suffers from the lack of materials capable of efficiently supporting osteogenesis. In this study, we developed 3D-printed porous scaffolds composed of polylactic acid/hydroxyapatite (PLA/HA) composites with high ceramic contents (above 20%, w/w) by FDM. The mechanical properties of the PLA/HA scaffolds were compatible with those of trabecular bone. In vitro degradation tests revealed that HA can neutralize the acidification effect caused by PLA degradation, while simultaneously releasing calcium and phosphate ions. Importantly, 3D-printed PLA/HA did not induce the upregulation of activation markers nor the expression of inflammatory cytokines in dendritic cells thus exhibiting no immune-stimulatory properties in vitro. Evaluations using human mesenchymal stem cells (MSC) showed that pure PLA scaffolds exerted an osteoconductive effect, whereas PLA/HA scaffolds efficiently induced osteogenic differentiation of MSC even in the absence of any classical osteogenic stimuli. Our findings indicate that 3D-printed PLA scaffolds loaded with high concentrations of HA are most suitable for future applications in bone tissue engineering. (AU)

• Processo FAPESP: 18/07860-9 - Desenvolvimento de filamentos nanocompósitos poliméricos com propriedades anti-inflamatórias para impressão 3D como substituintes ósseos
• Beneficiário: Marcela Piassi Bernardo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 19/23027-8 - Desenvolvimento e avaliação da interação celular de scaffolds impressos em 3D para reconstrução óssea
• Beneficiário: Marcela Piassi Bernardo
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado