hondroitin Sulfate Protects the Liver in an Experimental Model of Extra-Hepatic Cholestasis Induced by Common Bile Duct Ligatio

Autor(es)

Guedes, Pedro L. R. ^[1] ; Carvalho, Carolina P. F. ^[2] ; Carbonel, Adriana A. F. ^[3] ; Simoes, Manuel J. ^[4] ; Icimoto, Marcelo Y. ^[5] ; Aguiar, Jair A. K. ^[6] ; Kouyoumdjian, Maria ^[7] ; Gazarini, Marcos L. ^[2] ; Nagaoka, Marcia R. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Med, Escola Paulista Med, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biosci, Inst Saude Soc, BR-11015020 Santos, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Gynecol, Escola Paulista Med, BR-04039001 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Dept Morphol & Genet, Escola Paulista Med, BR-04023900 Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Dept Biophys, Escola Paulista Med, BR-04039032 Sao Paulo - Brazil

^[6] Univ Fed Juiz Fora, Dept Biochem, BR-36036900 Juiz De Fora - Brazil

^[7] Univ Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04023062 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: OLECULE; v. 27, n. 3 FEB 2022.
• Citações Web of Science: 0

Resumo

During liver fibrogenesis, there is an imbalance between regeneration and wound healing. The current treatment is the withdrawal of the causing agent; thus, investigation of new and effective treatments is important. Studies have highlighted the action of chondroitin sulfate (CS) in different cells; thus, our aim was to analyze its effect on an experimental model of bile duct ligation (BDL). Adult Wistar rats were subjected to BDL and treated with CS for 7, 14, 21, or 28 days intraperitoneally. We performed histomorphometric analyses on Picrosirius-stained liver sections. Cell death was analyzed according to caspase-3 and cathepsin B activity and using a TUNEL assay. Regeneration was evaluated using PCNA immunohistochemistry. BDL led to increased collagen content with corresponding decreased liver parenchyma. CS treatment reduced total collagen and increased parenchyma content after 21 and 28 days. The treatment also promoted changes in the hepatic collagen type III/I ratio. Furthermore, it was observed that CS treatment reduced caspase-3 activity and the percentage of TUNEL-positive cells after 14 days and cathepsin B activity only after 28 days. The regeneration increased after 14, 21, and 28 days of CS treatment. In conclusion, our study showed a promising hepatoprotective action of CS in fibrogenesis induced by BDL. (AU)

• Processo FAPESP: 08/55928-0 - Papel dos sistemas cinina e renina-angiotensina na lesão por isquemia e reperfusão de fígados de ratos
• Beneficiário: Márcia Regina Nagaoka
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/00896-5 - Citoproteção hepática: da fibrose a isquemia e reperfusão
• Beneficiário: Márcia Regina Nagaoka
• Modalidade de apoio: Auxílio à Pesquisa - Regular