iochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazi

Autor(es):Mostrar menos -

Aranha, Guilherme Mauro ^[1] ; Contato, Alex Graca ^[2] ; dos Santos Salgado, Jose Carlos ^[3] ; de Oliveira, Tassio Brito ^[1] ; Retamiro, Karina Miyuki ^[4] ; Ortolan, Guilherme Guimaraes ^[1] ; Crevelin, Eduardo Jose ^[3] ; Nakamura, Celso Vataru ^[4] ; Beraldo de Moraes, Luiz Alberto ^[3] ; Peralta, Rosane Marina ^[5] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Maringa, Dept Ciencias Basicas Saude, Maringa, Parana - Brazil

^[5] Univ Estadual Maringa, Dept Bioquim, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Microbiology; v. 53, n. 1, p. 349-358, MAR 2022.
• Citações Web of Science: 0

Resumo

The objective of this study was to evaluate the antioxidant activity, determine and quantify the phenolic compounds and other compounds, and evaluate the cellular cytotoxicity of mycelium extracts of two new Basidiomycete mushrooms strains isolated in Brazil and identified as Lepista sordida GMA-05 and Trametes hirsuta GMA-01. Higher amounts of proteins, free amino acids, total and reducing carbohydrates, and phenolic compounds as chlorogenic, ferulic, caffeic, and gallic acids were found in extracts of T. hirsuta and L. sordida. Protocatechuic acid was found only in aqueous extracts of L. sordida. The TLC of the extracts showed the predominance of glucose and smaller amounts of xylose. It was observed through UPLC-MS higher amounts of phenolic compounds. The aqueous extract from T. hirsuta had the most noteworthy results in the antioxidant assays, especially the ABTS test. The cytotoxic activity was evaluated using two different cell lineages and showed higher toxicity for L. sordida in macrophages J774-A1. However, in Vero cells, it was 12.6-fold less toxic when compared to T. hirsuta. Thus, both mushrooms show potential as functional foods or additives, presenting phenolic content, antioxidant activity, and low cytotoxic activity in the tested cells. (AU)

• Processo FAPESP: 18/07522-6 - Desenvolvimento de tecnologias ecológicas para a hidrólise de biomassa e resíduos da indústria de celulose e papel
• Beneficiário: Maria de Lourdes Teixeira de Moraes Polizeli
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/25862-6 - Estudos estruturais e propriedades funcionais de xiloglucanases e liquenases fúngicas: aplicações na hidrólise de biomassa lignocelulósica
• Beneficiário: Alex Graça Contato
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/50884-5 - INCT 2014: Instituto Nacional de Ciência e Tecnologia do Bioetanol
• Beneficiário: Marcos Silveira Buckeridge
• Modalidade de apoio: Auxílio à Pesquisa - Temático