iochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazi

Author(s):Show less -

Aranha, Guilherme Mauro ^[1] ; Contato, Alex Graca ^[2] ; dos Santos Salgado, Jose Carlos ^[3] ; de Oliveira, Tassio Brito ^[1] ; Retamiro, Karina Miyuki ^[4] ; Ortolan, Guilherme Guimaraes ^[1] ; Crevelin, Eduardo Jose ^[3] ; Nakamura, Celso Vataru ^[4] ; Beraldo de Moraes, Luiz Alberto ^[3] ; Peralta, Rosane Marina ^[5] ; Teixeira de Moraes Polizeli, Maria de Lourdes ^{[2, 1]}

Affiliation

^[1] Univ Sao Paulo, Dept Biol, Fac Filosofia Ciencias & Letras Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, Dept Quim, Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Maringa, Dept Ciencias Basicas Saude, Maringa, Parana - Brazil

^[5] Univ Estadual Maringa, Dept Bioquim, Maringa, Parana - Brazil

• Document type: Journal article
• Source: Brazilian Journal of Microbiology; v. 53, n. 1, p. 349-358, MAR 2022.
• Web of Science Citations: 0

Abstract

The objective of this study was to evaluate the antioxidant activity, determine and quantify the phenolic compounds and other compounds, and evaluate the cellular cytotoxicity of mycelium extracts of two new Basidiomycete mushrooms strains isolated in Brazil and identified as Lepista sordida GMA-05 and Trametes hirsuta GMA-01. Higher amounts of proteins, free amino acids, total and reducing carbohydrates, and phenolic compounds as chlorogenic, ferulic, caffeic, and gallic acids were found in extracts of T. hirsuta and L. sordida. Protocatechuic acid was found only in aqueous extracts of L. sordida. The TLC of the extracts showed the predominance of glucose and smaller amounts of xylose. It was observed through UPLC-MS higher amounts of phenolic compounds. The aqueous extract from T. hirsuta had the most noteworthy results in the antioxidant assays, especially the ABTS test. The cytotoxic activity was evaluated using two different cell lineages and showed higher toxicity for L. sordida in macrophages J774-A1. However, in Vero cells, it was 12.6-fold less toxic when compared to T. hirsuta. Thus, both mushrooms show potential as functional foods or additives, presenting phenolic content, antioxidant activity, and low cytotoxic activity in the tested cells. (AU)

• FAPESP's process: 18/07522-6 - Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry
• Grantee: Maria de Lourdes Teixeira de Moraes Polizeli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/25862-6 - Structural studies and functional properties of xyloglucanases and lichenases: applications on hydrolysis of lignocellulosic biomass
• Grantee: Alex Graça Contato
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol
• Grantee: Marcos Silveira Buckeridge
• Support Opportunities: Research Projects - Thematic Grants