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Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry


Brazil is the country with the highest biodiversity in the world. The microbiota present in different Brazilian biomes remains almost unknown and minimally studied, representing great potential for discovery, application in scientific research and industrial activities. Considering Brazil's great biodiversity, in 2010 the National Biodiversity Research System (SISBIOTA-Brazil) started isolating fungi from all Brazilian biomes, increasing the potential of obtaining different enzymes with biotechnological and industrial interests. Portugal and Brazil represent a large consumer market and producer of several compounds, but when it comes to enzymes, both countries still having a little expression. In the last 30 years it has been an huge increase in interest and research of enzymes that degrade lignocellulosic compounds, mainly due to waste surplus, for example, of the agro-industries, which generates tons of waste daily, which often become a problem of environment pollution. Therefore, it is necessary to maximize the use of organic matter, reducing the generation of waste and obtaining products with high added value. The lignocellulosic material consists mainly of three different types of compounds: cellulose, hemicellulose and lignin, which are associated to each other and in different proportions depending on the vegetable. The enzymatic conversion has been a method that is gaining prominence among the technology to convert lignocellulosic biomass into compounds with high added value. This process has mainly used xylanases, cellulases and ligninases. In this context, the present project has as an objective the evaluation of different eco-friendly technologies to pre-treat the agro-industrial/forest residues (such as residues from the pulp and paper industry: paper sludge and eucalyptus bark) aiming to facilitate the access of the fungal enzymes to the structural components. For this the physical-chemical pretreatments will be prioritized: ohmic heating and autohydrolysis, for the extraction of compounds derived from hemicellulose. At the same time, will be performed the screening of the filamentous fungi obtained in the SISBIOTA project using the residues from rice, coffee, beer, beans, maize and paper industries as inducer source. The enzymes produced will be characterized regarding their physicochemical parameters and immobilized in different substrates, aiming an improvement of the enzymatic hydrolysis of these residues. After that, an enzyme cocktail will be designed to be applied in the biological pre-treatment (enzymatic) of the lignocellulosic components (cellulose, hemicellulose and lignin), aiming to generate high-value products. The products obtained will be explored in the production of compounds with industrial interest, such as xylooligosaccharides, bioethanol and antioxidant/antimicrobial compounds. (AU)

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Scientific publications (15)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PASIN, THIAGO MACHADO; DE OLIVEIRA, TASSIO BRITO; DE ALMEIDA SCARCELLA, ANA SILVIA; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA; GUAZZARONI, MARIA-EUGENIA. Perspectives on Expanding the Repertoire of Novel Microbial Chitinases for Biological Control. Journal of Agricultural and Food Chemistry, v. 69, n. 11, p. 3284-3288, . (18/07522-6, 17/09000-4)
ALNOCH, ROBSON CARLOS; DOS SANTOS, LEANDRO ALVES; DE ALMEIDA, JANAINA MARQUES; KRIEGER, NADIA; MATEO, CESAR. Recent Trends in Biomaterials for Immobilization of Lipases for Application in Non-Conventional Media. CATALYSTS, v. 10, n. 6, . (20/00081-4, 18/07522-6)
ARANHA, GUILHERME MAURO; CONTATO, ALEX GRACA; DOS SANTOS SALGADO, JOSE CARLOS; DE OLIVEIRA, TASSIO BRITO; RETAMIRO, KARINA MIYUKI; ORTOLAN, GUILHERME GUIMARAES; CREVELIN, EDUARDO JOSE; NAKAMURA, CELSO VATARU; BERALDO DE MORAES, LUIZ ALBERTO; PERALTA, ROSANE MARINA; et al. iochemical characterization and biological properties of mycelium extracts from Lepista sordida GMA-05 and Trametes hirsuta GMA-01: new mushroom strains isolated in Brazi. Brazilian Journal of Microbiology, v. 53, n. 1, p. 349-358, . (18/07522-6, 17/25862-6, 14/50884-5)
DESAGIACOMO, CARLA CRISTINA VILLELA; ALNOCH, ROBSON CARLOS; PINHEIRO, VANESSA ELISA; CEREIA, MARIANA; MACHADO, CARLA BOTELHO; DAMASIO, ANDRE; AUGUSTO, MARLEI JOSIELE; PEDERSOLI, WELLINGTON; SILVA, ROBERTO NASCIMENTO; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Structural model and functional properties of an exo-polygalacturonase from Neosartorya glabra. International Journal of Biological Macromolecules, v. 186, p. 909-918, . (14/50884-5, 20/00081-4, 18/07522-6, 17/22669-0)
DE ALMEIDA, PAULA ZAGHETTO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; SANTOS SALGADO, JOSE CARLOS; PEREZ, MALENA MARTINEZ; GALAN, BEATRIZ; GARCIA, JOSE LUIS; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 beta-glucosidase from Anoxybacillus thermarum. Process Biochemistry, v. 99, p. 1-8, . (18/07522-6, 17/09000-4, 14/50884-5)
DE FREITAS, EMANUELLE N.; KHATRI, VINAY; CONTIN, DANIELE R.; DE OLIVEIRA, TASSIO B.; CONTATO, ALEX G.; PERALTA, ROSANE M.; DOS SANTOS, WANDERLEY D.; MARTINEZ, CARLOS A.; SADDLER, JACK N.; POLIZELI, MARIA DE LOURDES T. M.. limate change affects cell-wall structure and hydrolytic performance of a perennial grass as an energy cro. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 16, n. 2, . (08/58075-8, 08/57908-6, 17/23989-9, 19/07141-5, 18/07522-6)
SCARCELLA, ANA SILVIA DE ALMEIDA; PASIN, THIAGO MACHADO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; FERREIRA-NOZAWA, MONICA STROPA; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; BUCKERIDGE, MARCOS SILVEIRA; MICHELIN, MICHELE; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues. ENERGY, v. 226, . (14/50884-5, 18/07522-6, 17/09000-4)
PINHEIRO, VANESSA ELISA; HORVATH, ILONA SARVARI; LUNDIN, MAGNUS; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 15, n. 5, p. 1447-1460, . (18/07522-6, 14/50884-5)
DE FREITAS, EMANUELLE N.; KHATRI, VINAY; WU, JIE; TAKADA, MASATSUGU; SCARCELLA, ANA SILVIA DE A.; MARTINEZ, CARLOS ALBERTO; SADDLER, JACK N.; POLIZELI, MARIA DE LOURDES T. M.. Structural and compositional changes induced by hydrothermal and organosolv pretreatments impacts enzymatic hydrolysis of a tropical forage grass grown under future climate conditions. INDUSTRIAL CROPS AND PRODUCTS, v. 171, . (17/23989-9, 18/07522-6, 19/07141-5, 08/57908-6, 08/58075-8)
PINHEIRO, VANESSA ELISA; DE ALMEIDA, PAULA ZAGHETTO; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Statistical optimization of cornmeal saccharification using various hydrolases. BIOMASS CONVERSION AND BIOREFINERY, . (18/07522-6)
DE FREITAS, EMANUELLE NEIVERTH; ALNOCH, ROBSON CARLOS; CONTATO, ALEX GRACA; NOGUEIRA, V, KAROLINE MARIA; CREVELIN, EDUARDO JOSE; BERALDO DE MORAES, LUIZ ALBERTO; SILVA, ROBERTO NASCIMENTO; MARTINEZ, CARLOS ALBERTO; POLIZELI, MARIA DE LOURDES T. M.. Enzymatic Pretreatment with Laccases from Lentinus sajor-caju Induces Structural Modification in Lignin and Enhances the Digestibility of Tropical Forage Grass (Panicum maximum) Grown under Future Climate Conditions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 17, . (18/07522-6, 19/07141-5, 08/57908-6, 17/23989-9, 08/58075-8, 20/00081-4)
SOARES, GLEYDISON AMARANTE; ALNOCH, ROBSON CARLOS; SILVA DIAS, GLAUCO; SANTOS REIS, NADABE DOS; TAVARES, IASNAIA MARIA DE CARVALHO; RUIZ, HECTOR A.; BILAL, MUHAMMAD; DE OLIVEIRA, JULIETA RANGEL; KRIEGER, NADIA; FRANCO, MARCELO. Production of a fermented solid containing lipases from Penicillium roqueforti ATCC 10110 and its direct employment in organic medium in ethyl oleate synthesis. Biotechnology and Applied Biochemistry, . (18/07522-6)
PINHEIRO, VANESSA ELISA; WAINAINA, STEVEN; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES; HORVATH, ILONA SARVARI. Anaerobic digestion of cornmeal - the effect of crude enzyme extract and co-digestion with cow manure. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, . (14/50884-5, 18/07522-6)
CONTATO, ALEX GRACA; DE OLIVEIRA, TASSIO BRITO; ARANHA, GUILHERME MAURO; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; VIEIRA NOGUEIRA, KAROLINE MARIA; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; BUCKERIDGE, MARCOS SILVEIRA; SILVA, ROBERTO NASCIMENTO; et al. Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse. MICROORGANISMS, v. 9, n. 3, . (18/25898-3, 14/50884-5, 17/23989-9, 18/07522-6, 08/57908-6, 17/25862-6, 17/09000-4)

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