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Development of eco-friendly technologies for the hydrolysis of biomass and residues of cellulose pulp and paper industry

Abstract

Brazil is the country with the highest biodiversity in the world. The microbiota present in different Brazilian biomes remains almost unknown and minimally studied, representing great potential for discovery, application in scientific research and industrial activities. Considering Brazil's great biodiversity, in 2010 the National Biodiversity Research System (SISBIOTA-Brazil) started isolating fungi from all Brazilian biomes, increasing the potential of obtaining different enzymes with biotechnological and industrial interests. Portugal and Brazil represent a large consumer market and producer of several compounds, but when it comes to enzymes, both countries still having a little expression. In the last 30 years it has been an huge increase in interest and research of enzymes that degrade lignocellulosic compounds, mainly due to waste surplus, for example, of the agro-industries, which generates tons of waste daily, which often become a problem of environment pollution. Therefore, it is necessary to maximize the use of organic matter, reducing the generation of waste and obtaining products with high added value. The lignocellulosic material consists mainly of three different types of compounds: cellulose, hemicellulose and lignin, which are associated to each other and in different proportions depending on the vegetable. The enzymatic conversion has been a method that is gaining prominence among the technology to convert lignocellulosic biomass into compounds with high added value. This process has mainly used xylanases, cellulases and ligninases. In this context, the present project has as an objective the evaluation of different eco-friendly technologies to pre-treat the agro-industrial/forest residues (such as residues from the pulp and paper industry: paper sludge and eucalyptus bark) aiming to facilitate the access of the fungal enzymes to the structural components. For this the physical-chemical pretreatments will be prioritized: ohmic heating and autohydrolysis, for the extraction of compounds derived from hemicellulose. At the same time, will be performed the screening of the filamentous fungi obtained in the SISBIOTA project using the residues from rice, coffee, beer, beans, maize and paper industries as inducer source. The enzymes produced will be characterized regarding their physicochemical parameters and immobilized in different substrates, aiming an improvement of the enzymatic hydrolysis of these residues. After that, an enzyme cocktail will be designed to be applied in the biological pre-treatment (enzymatic) of the lignocellulosic components (cellulose, hemicellulose and lignin), aiming to generate high-value products. The products obtained will be explored in the production of compounds with industrial interest, such as xylooligosaccharides, bioethanol and antioxidant/antimicrobial compounds. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SCARCELLA, ANA SILVIA DE ALMEIDA; PASIN, THIAGO MACHADO; DE LUCAS, ROSYMAR COUTINHO; FERREIRA-NOZAWA, MONICA STROPA; DE OLIVEIRA, TASSIO BRITO; CONTATO, ALEX GRACA; GRANDIS, ADRIANA; BUCKERIDGE, MARCOS SILVEIRA; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties. BIOMASS CONVERSION AND BIOREFINERY, JAN 2021. Web of Science Citations: 0.
DE ALMEIDA, PAULA ZAGHETTO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; SANTOS SALGADO, JOSE CARLOS; PEREZ, MALENA MARTINEZ; GALAN, BEATRIZ; GARCIA, JOSE LUIS; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 beta-glucosidase from Anoxybacillus thermarum. Process Biochemistry, v. 99, p. 1-8, DEC 2020. Web of Science Citations: 0.
ALNOCH, ROBSON CARLOS; DOS SANTOS, LEANDRO ALVES; DE ALMEIDA, JANAINA MARQUES; KRIEGER, NADIA; MATEO, CESAR. Recent Trends in Biomaterials for Immobilization of Lipases for Application in Non-Conventional Media. CATALYSTS, v. 10, n. 6 JUN 2020. Web of Science Citations: 0.

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