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Utilization of residual lignocellulosic biomass from 2G ethanol processing to obtain nanocellulose via enzymatic route

Grant number: 18/10899-4
Support Opportunities:Scholarships in Brazil - Post-Doctorate
Effective date (Start): July 01, 2018
Effective date (End): February 02, 2022
Field of knowledge:Engineering - Chemical Engineering - Chemical Process Industries
Principal Investigator:Cristiane Sanchez Farinas
Grantee:Stanley Endrigo Bilatto Rodrigues
Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
Associated research grant:16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales, AP.BIOEN.TEM
Associated scholarship(s):19/24009-3 - Nanocellulose-conducting aerogels for thermoelectric application, BE.EP.PD

Abstract

In the second generation (2G) ethanol production process by the enzymatic route, complete saccharification of the cellulose structure is not achieved, especially in processes with high solids loading. This residual solid fraction of the saccharification process of the cellulose is a material with crystalline characteristic, which allows its use in the form of nanocellulose. Nanocellulose, in the form of nanofibers or nanocrystals, has applications in different industrial sectors, such as cosmetics, medicine, packaging industry, biocatalysts, among others. In this postdoctoral proposal, it is proposed the development of a bioprocess to obtain nanocellulose from the residue of the enzymatic hydrolysis, with the substrate derived from sugarcane (straw and bagasse). Different enzymatic cocktails and reactional conditions will be evaluated, as well as the association of mechanical processes aiming at obtaining nanocellulose exclusively by technologies inserted in green chemistry concepts. Thus, in this project the following steps will be performed: 1) obtaining the nanocellulose; 2) physicochemical characterization of the nanomaterials; and 3) evaluating possible applications for this nanomaterial. This will involve both the area of bioprocesses, for optimization of enzymatic cocktails, reactive conditions, more appropriate bioreactors and the association of mechanical treatment, as well as the physical-chemical characterization area of the materials. The evaluation of the application potential of the nanocellulose obtained in three products of technological interest will also be part of the activities foreseen in this postdoctoral proposal. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LEITE, LILIANE S. F.; BILATTO, STANLEY; PASCHOALIN, RAFAELLA T.; SOARES, ANDREY C.; MOREIRA, V, FRANCYS K.; OLIVEIRA, JR., OSVALDO N.; MATTOSO, LUIZ H. C.; BRAS, JULIEN. Eco-friendly gelatin films with rosin-grafted cellulose nanocrystals for antimicrobial packaging. International Journal of Biological Macromolecules, v. 165, n. B, p. 2974-2983, . (18/22214-6, 18/10899-4, 18/00278-2, 17/18725-2, 18/18953-8)
BILATTO, STANLEY; MARCONCINI, JOSE M.; MATTOSO, LUIZ H. C.; FARINAS, CRISTIANE S.. Lignocellulose nanocrystals from sugarcane straw. INDUSTRIAL CROPS AND PRODUCTS, v. 157, . (18/10899-4, 16/10636-8)
SQUINCA, PAULA; BILATTO, STANLEY; BADINO, ALBERTO C.; FARINAS, CRISTIANE S.. Nanocellulose Production in Future Biorefineries: An Integrated Approach Using Tailor-Made Enzymes. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 8, n. 5, p. 2277-2286, . (18/10899-4, 16/10636-8)
FREITAS, V, JULIANA; BILATTO, STANLEY; SQUINCA, PAULA; PINTO, ARIANE S. S.; BRONDI, MARIANA G.; BONDANCIA, THALITA J.; BATISTA, GUSTAVO; KLAIC, RODRIGO; FARINAS, CRISTIANE S.. Sugarcane biorefineries: potential opportunities towards shifting from wastes to products. INDUSTRIAL CROPS AND PRODUCTS, v. 172, . (16/10636-8, 19/25261-8, 17/21173-1, 18/10899-4)
LEITE, LILIANE SAMARA FERREIRA; PHAM, CYNTHIA; BILATTO, STANLEY; AZEREDO, HENRIETTE M. C.; CRANSTON, EMILY D.; MOREIRA, FRANCYS KLEY; MATTOSO, LUIZ HENRIQUE C.; BRAS, JULIEN. Effect of Tannic Acid and Cellulose Nanocrystals on Antioxidant and Antimicrobial Properties of Gelatin Films. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 9, n. 25, p. 8539-8549, . (18/00278-2, 18/10899-4)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.