Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanocellulose Production in Future Biorefineries: An Integrated Approach Using Tailor-Made Enzymes

Full text
Author(s):
Squinca, Paula [1, 2] ; Bilatto, Stanley [1] ; Badino, Alberto C. [2] ; Farinas, Cristiane S. [1, 2]
Total Authors: 4
Affiliation:
[1] Embrapa Instrumentat, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos - Brazil
[2] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 8, n. 5, p. 2277-2286, FEB 10 2020.
Web of Science Citations: 8
Abstract

The development of process engineering approaches to integrate the production of biofuels and high value-added biobased products, such as enzymes and nanocellulose, is crucial to improve the financial performance and sustainability of lignocellulosic biomass biorefineries. Here, the feasibility of applying enzymes produced on-site to obtain nanocellulose was evaluated using eucalyptus cellulose pulp as a model feedstock. A systematic analysis of the structural properties of the nanomaterials obtained after hydrolysis using a cellulolytic enzymatic complex with high endoglucanase specific activity (17.09 IU/mg(protein)), produced by Aspergillus niger, followed by sonication, revealed that longer ball milling pretreatment and reaction times favored extraction of the cellulose nanocrystals (CNCs). The highest yield (24.6%) of CNCs was achieved using 96 h of enzymatic hydrolysis of the ball-milled cellulose pulp, followed by sonication for 5 min. The CNCs presented approximate lengths of 294.0 nm and diameters of 24.0 nm, and the crystallinity index increased from 57.5% to 78.3%, compared to the cellulose pulp that was only ball milled. These findings demonstrated that nanocelluloses could be successfully extracted using on-site produced enzymes and that the sustainable integrated process reported here could contribute to the development of the nascent biobased economy. (AU)

FAPESP's process: 18/10899-4 - Utilization of residual lignocellulosic biomass from 2G ethanol processing to obtain nanocellulose via enzymatic route
Grantee:Stanley Endrigo Bilatto Rodrigues
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support type: Program for Research on Bioenergy (BIOEN) - Thematic Grants