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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nanocellulose Production in Future Biorefineries: An Integrated Approach Using Tailor-Made Enzymes

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Squinca, Paula [1, 2] ; Bilatto, Stanley [1] ; Badino, Alberto C. [2] ; Farinas, Cristiane S. [1, 2]
Total Authors: 4
[1] Embrapa Instrumentat, Nanotechnol Natl Lab Agr LNNA, BR-13560970 Sao Carlos - Brazil
[2] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos - Brazil
Total Affiliations: 2
Document type: Journal article
Source: ACS SUSTAINABLE CHEMISTRY & ENGINEERING; v. 8, n. 5, p. 2277-2286, FEB 10 2020.
Web of Science Citations: 8

The development of process engineering approaches to integrate the production of biofuels and high value-added biobased products, such as enzymes and nanocellulose, is crucial to improve the financial performance and sustainability of lignocellulosic biomass biorefineries. Here, the feasibility of applying enzymes produced on-site to obtain nanocellulose was evaluated using eucalyptus cellulose pulp as a model feedstock. A systematic analysis of the structural properties of the nanomaterials obtained after hydrolysis using a cellulolytic enzymatic complex with high endoglucanase specific activity (17.09 IU/mg(protein)), produced by Aspergillus niger, followed by sonication, revealed that longer ball milling pretreatment and reaction times favored extraction of the cellulose nanocrystals (CNCs). The highest yield (24.6%) of CNCs was achieved using 96 h of enzymatic hydrolysis of the ball-milled cellulose pulp, followed by sonication for 5 min. The CNCs presented approximate lengths of 294.0 nm and diameters of 24.0 nm, and the crystallinity index increased from 57.5% to 78.3%, compared to the cellulose pulp that was only ball milled. These findings demonstrated that nanocelluloses could be successfully extracted using on-site produced enzymes and that the sustainable integrated process reported here could contribute to the development of the nascent biobased economy. (AU)

FAPESP's process: 18/10899-4 - Utilization of residual lignocellulosic biomass from 2G ethanol processing to obtain nanocellulose via enzymatic route
Grantee:Stanley Endrigo Bilatto Rodrigues
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants