Development of bioprocesses for obtention of products with potential applications in bioenergy and feed industries using agro-industrial residues

• Grant number: 14/24188-1
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Regular Program Grants
• Start date: March 01, 2015
• End date: August 31, 2017
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Pedro de Oliva Neto
• Grantee: Pedro de Oliva Neto
• Host Institution: Faculdade de Ciências e Letras (FCL-ASSIS). Universidade Estadual Paulista (UNESP). Campus de Assis. Assis , SP, Brazil
• Associated researchers: Ana Flávia Azevedo Carvalho ; Bruna Escaramboni

Abstract

The technological use of lignocellulolitic agroindustrial waste has been much interest worldwide due to be a new renewable source for the production of food, chemicals and biofuels. On the national scene there is a great quantity of waste available, among which stand out the sugarcane bagasse, cassava bagasse, wheat bran and citrus pulp, from agricultural activities. These raw materials can serve as a substrate for the production of products with high added value, through bioprocesses performed by selected microorganisms. Furthermore, they consist of abundant source of complex carbohydrates like cellulose, hemicelluloses and starch but low protein content. The challenge is to obtain fermentable sugars from these wastes through safe and economical bioprocesses. The development of bioprocesses including the definition of microorganisms, cultivation parameters, formulation of economic and efficient medium of culture using lignocellulosic wastes and production optimization , separation and purification of biomolecules add value to these processes are important goals for success the application of this research in conversion of waste to bioproducts industries of food, feed and energy. The knowledge of the hydrolytic enzyme complexes with including cellulases, xylanases and amylases are also reactions is critical to success in the degradation of cellulose , hemicellulose and starch, respectively. The several applications of these enzymes are varied and can be found in the bioenergy, production of functional foods, pulp and paper, animal feed and fine chemical sectors. Another possibility is the conversion of lignocellulosic residues into unicellular protein by special yeasts. The implementation of integrated bioprocesses is possible for the production of enzymes, sugars and ethanol, with reduced investments due to synergies of infrastructure already in place, thus avoiding logistics costs due to the production "in loco". In this research project are proposed the study of some bioprocesses for the production of carbohydrases, sugars and proteins in order to contribute to the development of new technologies for supply nutrients and biofuels from agricultural residues. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ESCARAMBONI, BRUNA; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO; AZEVEDO CARVALHO, ANA FLAVIA; DE OLIVA NETO, PEDRO. Ethanol biosynthesis by fast hydrolysis of cassava bagasse using fungal amylases produced in optimized conditions. INDUSTRIAL CROPS AND PRODUCTS, v. 112, p. 368-377, FEB 2018. (12/16651-8, 14/24188-1)

SAKITA, GABRIEL ZANUTO; VENTOSO BOMPADRE, THIAGO FRANCISCO; DINESHKUMAR, DHANASEKARAN; TAVARES LIMA, PAULO DE MELLO; ABDALLA FILHO, ADIBE LUIZ; CAMPIONI, TANIA SILA; DE OLIVA NETO, PEDRO; BREMER NETO, HERMAN; LOUVANDINI, HELDER; ABDALLA, ADIBE LUIZ. Fibrolytic enzymes improving in vitro rumen degradability of tropical forages. JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION, v. 104, n. 5, APR 2020. (14/24188-1)

DE BARROS RANKE, FABIANE FERNANDA; SHINYA, THAIS YUMI; DE FIGUEIREDO, FRANCIANE CRISTINA; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO; CABRAL, HAMILTON; NETO, PEDRO DE OLIVA. Ethanol from rice byproduct using amylases secreted by Rhizopus microsporus var. oligosporus. Enzyme partial purification and characterization. Journal of Environmental Management, v. 266, JUL 15 2020. (14/24188-1)

SHINYA, THAIS YUMI; PEREIRA ELSNER, VICTOR HENRIQUE; DE LIMA JUNIOR, DAVID SPRESSAO; DE BARROS RANKE, FABIANE FERNANDA; ESCARAMBONI, BRUNA; DA PAIXAO MELO, WEILAN GOMES; FERNANDEZ NUNEZ, EUTIMIO GUSTAVO; NETO, PEDRO DE OLIVA. Bioprocess development with special yeasts for cassava bagasse enrichment nutritional to use in animal feed. ANIMAL FEED SCIENCE AND TECHNOLOGY, v. 290, p. 17-pg., 2022-06-04. (14/24188-1)

Filed patent(s) as a result of this research project