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INCT 2014: National Institute of Science and Technology of Bioethanol

Abstract

During the second phase of the INCT-bioethanol we intend to integrate the knowledge obtained during the last 6 years to assemble enzyme consortia that will hydrolyze the cell walls of sugarcane quickly and efficiently. The main focus will be to design enzyme consortiums that can be used to hydrolyze diverse sugarcane biomasses as well as genetically and chemically transformed varieties. The aim is to define combinations of enzymes that can improve the hydrolysis process by identifying different patterns of correspondence between variations in cell wall composition and different enzyme consortia. The consortia will be assembled from the 70 or so different hydrolases available in the INCT collection. In order to achieve this integration, a high throughput saccharification system will be employed to run analyses initially with commercial enzyme cocktails. Samples with different levels of sugarcane biomass saccharification will be further analyzed in order to understand the relationship between the cell wall chemical variability and degree of hydrolysis. This will enable the identification of cell wall-related genetic markers that can be used to enhance existing varieties and direct the search for new varieties of sugarcane. Simultaneously, genes encoding cell wall hydrolases will be silenced or activated using an INCT collection of genes present in the genome of sugarcane. Manipulating these genes, will be possible to evaluate the respective impacts of hydrolysis on three major limiting factors to hydrolysis: porosity, glycomic code and macrofibrilar structure of biomass. The objective is to produce, in the long term, sugarcane plants that display internal mechanisms designed to facilitate hydrolysis, possibly coupled with controlled activation mechanism that induce plants to express specific hydrolases. The implications of gaining the knowledge to control the composition and turnover of cell walls go beyond the development of technologies for ethanol production. By controlling the process of cell wall formation and deposition, possibilities such as the use of sugarcane mills as true biorefineries become feasible. One example is the impact that the use of lignans or hemicellulosic polysaccharides, such as glucan and xyloglucan, may have on their use as food additives, in cosmetics and for medical applications. Therefore, the knowledge that will be produced by the INCT-bioethanol in this second phase will significantly strengthen the Brazilian position as a developer of novel biomaterials-based products further elevating the sustainability of the sugarcane system. It should be emphasized that the consolidation of procedures resulting in high efficiency hydrolysis in sugarcane will have a positive impact in the development of this type of process in other parts of the world, since cell walls of other grasses used for bioenergy production (e.g. maize, rice, sorghum and miscanthus) are in many ways similar. For this reason, this project will strengthen the Brazilian strategic goal to achieve a leading position as an exporter of 2G technologies to other countries. It is noteworthy that the INCT-bioethanol, already has the information to design strategies for sugarcane engineering to produce cell walls that are more amenable to hydrolysis. This type of technology opens the way for the engineering of other grasses of significant agronomic value. (AU)

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Scientific publications (33)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FURTADO, GILVAN PESSOA; CARLI, SIBELI; MELEIRO, LUANA PARRAS; SANTOS SALGADO, JOSE CARLOS; WARD, RICHARD JOHN. Enhanced hydrolytic efficiency of an engineered CBM11-glucanase enzyme chimera against barley beta-D-glucan extracts. Food Chemistry, v. 365, DEC 15 2021. Web of Science Citations: 0.
DESAGIACOMO, CARLA CRISTINA VILLELA; ALNOCH, ROBSON CARLOS; PINHEIRO, VANESSA ELISA; CEREIA, MARIANA; MACHADO, CARLA BOTELHO; DAMASIO, ANDRE; AUGUSTO, MARLEI JOSIELE; PEDERSOLI, WELLINGTON; SILVA, ROBERTO NASCIMENTO; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Structural model and functional properties of an exo-polygalacturonase from Neosartorya glabra. International Journal of Biological Macromolecules, v. 186, p. 909-918, SEP 1 2021. Web of Science Citations: 0.
BECACICI MACIEIRA, BERNARDO PRETTI; LOCOSSELLI, GIULIANO MASELLI; BUCKERIDGE, MARCOS SILVEIRA; JARDIM, VINICIUS CARVALHO; KROTTENTHALER, STEFAN; ANHUF, DIETER; HELLE, GERHARD; FAUSTINI CUZZUOL, GERALDO ROGERIO; CECCANTINI, GREGORIO. Will climate change shift carbon allocation and stem hydraulics? Insights on a systemic view of carbon- and water-related wood traits in an anysohydric tropical tree species (Hymenaea courbaril, Leguminosae). ECOLOGICAL INDICATORS, v. 128, SEP 2021. Web of Science Citations: 0.
CARLI, SIBELI; SALGADO, JOSE CARLOS SANTOS; MELEIRO, LUANA PARRAS; WARD, RICHARD JOHN. Covalent Immobilization of Chondrostereum purpureum Endopolygalacturonase on Ferromagnetic Nanoparticles: Catalytic Properties and Biotechnological Application. Applied Biochemistry and Biotechnology, SEP 2021. Web of Science Citations: 0.
NAVARRO, V, BRUNO; ELBL, PAULA; DE OLIVEIRA, LEANDRO F.; PIOVEZANI, AMANDA R.; DOS SANTOS, ANDRE L. W.; DE SOUZA, DIEGO T.; DEMARCO, DIEGO; BUCKERIDGE, MARCOS S.; FLOH, ENY I. S. Cell-to-cell trafficking patterns in cell lines of Araucariaangustifolia (Brazilian pine) with contrasting embryogenic potential. PLANT CELL TISSUE AND ORGAN CULTURE, SEP 2021. Web of Science Citations: 0.
NEGRAO, DJANIRA R.; GRANDIS, ADRIANA; BUCKERIDGE, MARCOS S.; ROCHA, GEORGE J. M.; LEAL, V, MANOEL REGIS L.; DRIEMEIER, CARLOS. Inorganics in sugarcane bagasse and straw and their impacts for bioenergy and biorefining: A review. RENEWABLE & SUSTAINABLE ENERGY REVIEWS, v. 148, SEP 2021. Web of Science Citations: 0.
PINHEIRO, VANESSA ELISA; HORVATH, ILONA SARVARI; LUNDIN, MAGNUS; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, v. 15, n. 5, p. 1447-1460, SEP 2021. Web of Science Citations: 1.
SCARCELLA, ANA SILVIA DE ALMEIDA; PASIN, THIAGO MACHADO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; FERREIRA-NOZAWA, MONICA STROPA; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; BUCKERIDGE, MARCOS SILVEIRA; MICHELIN, MICHELE; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues. ENERGY, v. 226, JUL 1 2021. Web of Science Citations: 0.
PINHEIRO, VANESSA ELISA; HORVATH, ILONA SARVARI; LUNDIN, MAGNUS; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy. BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR, JUN 2021. Web of Science Citations: 0.
PEREIRA, GUILHERME MARTINS; DA SILVA CAUMO, SOFIA ELLEN; GRANDIS, ADRIANA; MOTA DO NASCIMENTO, EMERSON QUEIROZ; CORREIA, ALEXANDRE LIMA; JORGE BARBOSA, HENRIQUE DE MELO; MARCONDES, MARTA ANGELA; BUCKERIDGE, MARCOS SILVEIRA; VASCONCELLOS, PEROLA DE CASTRO. Physical and chemical characterization of the 2019 ``black rain{''} event in the Metropolitan Area of Sao Paulo, Brazil. Atmospheric Environment, v. 248, MAR 1 2021. Web of Science Citations: 0.
CONTATO, ALEX GRACA; DE OLIVEIRA, TASSIO BRITO; ARANHA, GUILHERME MAURO; DE FREITAS, EMANUELLE NEIVERTH; VICI, ANA CLAUDIA; VIEIRA NOGUEIRA, KAROLINE MARIA; DE LUCAS, ROSYMAR COUTINHO; DE ALMEIDA SCARCELLA, ANA SILVIA; BUCKERIDGE, MARCOS SILVEIRA; SILVA, ROBERTO NASCIMENTO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse. MICROORGANISMS, v. 9, n. 3 MAR 2021. Web of Science Citations: 1.
GRANDIS, ADRIANA; ARENQUE-MUSA, BRUNA C.; MARTINS, MARINA C. M.; MACIEL, THAIS OLIVAR; SIMISTER, RACHAEL; GOMEZ, LEONARDO D.; BUCKERIDGE, MARCOS S. Senna reticulata: a Viable Option for Bioenergy Production in the Amazonian Region. BioEnergy Research, v. 14, n. 1, p. 91-105, MAR 2021. Web of Science Citations: 0.
SCARCELLA, ANA SILVIA DE ALMEIDA; PASIN, THIAGO MACHADO; DE LUCAS, ROSYMAR COUTINHO; FERREIRA-NOZAWA, MONICA STROPA; DE OLIVEIRA, TASSIO BRITO; CONTATO, ALEX GRACA; GRANDIS, ADRIANA; BUCKERIDGE, MARCOS SILVEIRA; POLIZELI, MARIA DE LOURDES TEIXEIRA DE MORAES. Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties. BIOMASS CONVERSION AND BIOREFINERY, JAN 2021. Web of Science Citations: 0.
PINHEIRO, P. MATHEUS; REIS, A. G. RENATA; DUPREE, PAUL; WARD, J. RICHARD. Plant cell wall architecture guided design of CBM3-GH11 chimeras with enhanced xylanase activity using a tandem repeat left-handed beta-3-prism scaffold. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v. 19, p. 1108-1118, 2021. Web of Science Citations: 0.
PAGLIUSO, DEBORA; PALACIOS JARA, CARMEN EUSEBIA; GRANDIS, ADRIANA; LAM, ERIC; PENA FERREIRA, MARCELO JOSE; BUCKERIDGE, MARCOS SILVEIRA. Flavonoids from duckweeds: potential applications in the human diet. RSC ADVANCES, v. 10, n. 73, p. 44981-44988, DEC 20 2020. Web of Science Citations: 0.
SIMOES, MARCELLA SIQUEIRA; FERREIRA, SAVIO SIQUEIRA; GRANDIS, ADRIANA; RENCORET, JORGE; PERSSON, STAFFAN; FLOH, ENY IOCHEVET SEGAL; FERRAZ, ANDRE; DEL RIO, JOSE C.; BUCKERIDGE, MARCOS SILVEIRA; CESARINO, IGOR. Differentiation of Tracheary Elements in Sugarcane Suspension Cells Involves Changes in Secondary Wall Deposition and Extensive Transcriptional Reprogramming. FRONTIERS IN PLANT SCIENCE, v. 11, DEC 18 2020. Web of Science Citations: 1.
DE ALMEIDA, PAULA ZAGHETTO; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; SANTOS SALGADO, JOSE CARLOS; PEREZ, MALENA MARTINEZ; GALAN, BEATRIZ; GARCIA, JOSE LUIS; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Heterologous production and biochemical characterization of a new highly glucose tolerant GH1 beta-glucosidase from Anoxybacillus thermarum. Process Biochemistry, v. 99, p. 1-8, DEC 2020. Web of Science Citations: 0.
BERNARDES, WILLIAN SOUZA; MENOSSI, MARCELO. Plant 3' Regulatory Regions From mRNA-Encoding Genes and Their Uses to Modulate Expression. FRONTIERS IN PLANT SCIENCE, v. 11, AUG 14 2020. Web of Science Citations: 1.
GRANDIS, ADRIANA; ARENQUE-MUSA, BRUNA C.; MARTINS, MARINA C. M.; MACIEL, THAIS OLIVAR; SIMISTER, RACHAEL; GOMEZ, LEONARDO D.; BUCKERIDGE, MARCOS S. Senna reticulata: a Viable Option for Bioenergy Production in the Amazonian Region. BioEnergy Research, v. 14, n. 1 AUG 2020. Web of Science Citations: 0.
PASIN, THIAGO MACHADO; SANTOS SALGADO, JOSE CARLOS; DE ALMEIDA SCARCELLA, ANA SILVIA; DE OLIVEIRA, TASSIO BRITO; DE LUCAS, ROSYMAR COUTINHO; CEREIA, MARIANA; ROSA, JOSE CESAR; WARD, RICHARD JOHN; BUCKERIDGE, MARCOS SILVEIRA; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. A Halotolerant Endo-1,4-beta-Xylanase fromAspergillus clavatuswith Potential Application for Agroindustrial Residues Saccharification. Applied Biochemistry and Biotechnology, v. 191, n. 3, p. 1111-1126, JUL 2020. Web of Science Citations: 1.
SPIROPULOS GONCALVES, ENRICO CERIONI; PEREZ, MALENA MARTINEZ; VICI, ANA CLAUDIA; SANTOS SALGADO, JOSE CARLOS; ROCHA, MARIANA DE SOUZA; DE ALMEIDA, PAULA ZAGHETTO; INFANTE, JULIANA DA CONCEICAO; DE ALMEIDA SCARCELLA, ANA SILVIA; DE LUCAS, ROSYMAR COUTINHO; VIEIRA, ANDRESSA TIRONI; DE FARIA, ANIZIO MARCIO; FERREIRA BATISTA, ANTONIO CARLOS; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Potential biodiesel production from Brazilian plant oils and spent coffee grounds by Beauveria bassiana lipase 1 expressed in Aspergillus nidulans A773 using different agroindustry inputs. JOURNAL OF CLEANER PRODUCTION, v. 256, MAY 20 2020. Web of Science Citations: 5.
OLIVEIRA MONTEIRO, LUMMY MARIA; VICI, ANA CLAUDIA; PINHEIRO, MATHEUS PINTO; HEINEN, PAULO RICARDO; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; WARD, RICHARD JOHN; PRADE, ROLF ALEXANDER; BUCKERIDGE, MARCOS S.; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. A Highly Glucose Tolerant ss-Glucosidase from Malbranchea pulchella (MpBg3) Enables Cellulose Saccharification. SCIENTIFIC REPORTS, v. 10, n. 1 APR 24 2020. Web of Science Citations: 0.
DE PINHO TAVARES, EVELINE QUEIROZ; MATTOS MARTINS, MARINA CAMARA; GRANDIS, ADRIANA; ROMIM, GRAYCE H.; PIOVEZANI, AMANDA RUSISKA; GAIARSA, JONAS WEISSMANN; BUCKERIDGE, MARCOS SILVEIRA. Newly identified miRNAs may contribute to aerenchyma formation in sugarcane roots. PLANT DIRECT, v. 4, n. 3 MAR 2020. Web of Science Citations: 1.
GRANDIS, ADRIANA; LEITE, DEBORA C. C.; TAVARES, EVELINE Q. P.; ARENQUE-MUSA, BRUNA C.; GAIARSA, JONAS W.; MARTINS, MARINA C. M.; DE SOUZA, AMANDA P.; GOMEZ, LEONARDO D.; FABBRI, CLAUDIA; MATTEI, BENEDETTA; BUCKERIDGE, MARCOS S. Cell wall hydrolases act in concert during aerenchyma development in sugarcane roots. ANNALS OF BOTANY, v. 124, n. 6, SI, p. 1067-1089, NOV 4 2019. Web of Science Citations: 1.
PINHEIRO, VANESSA ELISA; MICHELIN, MICHELE; VICI, ANA CLAUDIA; DE ALMEIDA, PAULA ZAGHETTO; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Trametes versicolor laccase production using agricultural wastes: a comparative study in Erlenmeyer flasks, bioreactor and tray. Bioprocess and Biosystems Engineering, v. 43, n. 3 NOV 2019. Web of Science Citations: 2.
DE ALMEIDA SCARCELLA, ANA SILVIA; SOMERA, ALEXANDRE FAVARIN; CARREIRA NUNES, CHRISTIANE DA COSTA; GOMES, ELENI; VICI, ANA CLAUDIA; BUCKERIDGE, MARCOS SILVEIRA; DE MORAES POLIZELI, MARIA DE LOURDES TEIXEIRA. Matrix Discriminant Analysis Evidenced Surface-Lithium as an Important Factor to Increase the Hydrolytic Saccharification of Sugarcane Bagasse. Molecules, v. 24, n. 19 OCT 2019. Web of Science Citations: 0.
NEGRAO, DJANIRA R.; LING, LIU Y.; BORDONAL, RICARDO O.; DRIEMEIER, CARLOS. Microscale Analyses of Mineral Particles in Sugar Cane Bagasse and Straw Shed Light on How Debris Can Be Incorporated into Biomass. ENERGY & FUELS, v. 33, n. 10, p. 9965-9973, OCT 2019. Web of Science Citations: 0.
OLIVEIRA MONTEIRO, LUMMY MARIA; PEREIRA, MARITA GIMENEZ; VICI, ANA CLAUDIA; HEINEN, PAULO RICARDO; BUCKERIDGE, MARCOS S.; TEIXEIRA DE MORAES POLIZELI, MARIA DE LOURDES. Efficient hydrolysis of wine and grape juice anthocyanins by Malbranchea pulchella beta-glucosidase immobilized on MANAE-agarose and ConA-Sepharose supports. International Journal of Biological Macromolecules, v. 136, p. 1133-1141, SEP 1 2019. Web of Science Citations: 0.
JARDIM, VINICIUS CARVALHO; SANTOS, SUZANA DE SIQUEIRA; FUJITA, ANDRE; BUCKERIDGE, MARCOS SILVEIRA. BioNetStat: A Tool for Biological Networks Differential Analysis. FRONTIERS IN GENETICS, v. 10, JUN 21 2019. Web of Science Citations: 0.
TAVARES, EVELINE Q. P.; DE SOUZA, AMANDA P.; ROMIM, GRAYCE H.; GRANDIS, ADRIANA; PLASENCIA, ANNA; GAIARSA, JONAS W.; GRIMA-PETTENATI, JACQUELINE; DE SETTA, NATHALIA; VAN SLUYS, MARIE-ANNE; BUCKERIDGE, MARCOS S. The control of endopolygalacturonase expression by the sugarcane RAV transcription factor during aerenchyma formation. Journal of Experimental Botany, v. 70, n. 2, p. 497-506, JAN 15 2019. Web of Science Citations: 1.
DRIEMEIER, CARLOS E.; LING, LIU Y.; YANCY-CABALLERO, DAISON; MANTELATTO, PAULO E.; DIAS, CARLOS S. B.; ARCHILHA, NATHALY L. Location of water in fresh sugarcane bagasse observed by synchrotron X-ray microtomography. PLoS One, v. 13, n. 12 DEC 6 2018. Web of Science Citations: 2.
SANTOS SALGADO, JOSE CARLOS; MELEIRO, LUANA PARRAS; CARLI, SIBELI; WARD, RICHARD JOHN. Glucose tolerant and glucose stimulated beta-glucosidases - A review. Bioresource Technology, v. 267, p. 704-713, NOV 2018. Web of Science Citations: 15.
FONSECA-MALDONADO, RAQUEL; MELEIRO, LUANA P.; MENDES, LUIS F. S.; ALVES, LUANA F.; CARLI, SIBELI; MORERO, LUCAS D.; BASSO, LUIS G. M.; COSTA-FILHO, ANTONIO J.; WARD, RICHARD J. Lignocellulose binding of a Cel5A-RtCBM11 chimera with enhanced beta-glucanase activity monitored by electron paramagnetic resonance. BIOTECHNOLOGY FOR BIOFUELS, v. 10, NOV 14 2017. Web of Science Citations: 4.

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