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Immobilization and co-immobilization of lignocellulolytic enzymes in magnetic nanoparticles: a strategy for hydrolysis of biomass and industry waste

Grant number: 20/15510-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): December 01, 2021
Effective date (End): November 30, 2023
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal researcher:Maria de Lourdes Teixeira de Moraes Polizeli
Grantee:Diandra de Andrades
Home Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:14/50884-5 - INCT 2014: National Institute of Science and Technology of Bioethanol, AP.TEM

Abstract

Lignocellulosic biomass is an abundant resource composed mainly of cellulose, hemicellulose and lignin polymers. These polymers are a renewable energy source for the production of biofuels and other compounds with high added value. However, its components are not readily available for bioconversion, and a set of enzymes is required its to deconstruct. Enzymatic application is still limited and expensive, and the immobilization of these biocatalysts is an important tool for obtaining more efficient and economically viable biocatalysts. Thus, the purpose of this study is to immobilize different biomass degrading enzymes using the technology of magnetic nanoparticles combined with nanocellulose to obtain a biocatalyst easy to reuse and separation from the reaction medium with high performance in the production of fermentable sugars to ethanol and other compounds of value. So, residues will be used for the production of nanocellulose and subsequently used in the preparation of magnetic nanoparticles. In the search for the most stable and efficient biocatalyst, the coating of the particles with chitosan and the crosslinking of the derivatives with the bifunctional agents glutaraldehyde and genipine will also be tested. The best derivatives will be compared for reuse, thermal and operational stability in the production of the compounds of interest. Finally, we hope to develop an innovative multicatalyst combining the advantages of using magnetic nanoparticles with multilayer immobilization technology, favoring the synergy of these enzymes in waste hydrolysis. Thus, we will be serving the purposes of the two LMBC projects, the bioethanol INCT, CNPq process nº 465319/2014-9/FAPESP nº 2014/50884-5, and the Cooperation Agreements/FCT - Foundation for Science and Technology of Portugal/São Paulo State Research Support Foundation, FAPESP process: 2018/07522-6. (AU)

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