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National Institute of Science and Technology of Bioethanol

Grant number: 08/57908-6
Support type:Program for Research on Bioenergy (BIOEN) - Thematic Grants
Duration: March 01, 2009 - February 28, 2015
Field of knowledge:Biological Sciences - Botany
Cooperation agreement: CNPq - INCTs
Principal Investigator:Marcos Silveira Buckeridge
Grantee:Marcos Silveira Buckeridge
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated grant(s):11/12611-9 - Sugarcane metabolomics, AV.EXT
Associated scholarship(s):12/15281-2 - Molecular, cytogenetic and phenotypic characterization of accessions from the Saccharum complex for genetic introgression, BP.DR
11/02344-3 - Effects of elevated CO2 and water deficit in sugarcane metabolism and physiology: a system biology approach, BP.PD
10/12833-9 - Characterization of cell wall degradation in the aerenchyma formation in roots of sugarcane, BP.MS
+ associated scholarships 10/13579-9 - Sugarcane antioxidative system in response to drought, BP.MS
10/10184-3 - Development of an enzymatic cocktail for application in biorefinery, BP.DR
10/07133-8 - Creation of an allosteric endoxylanase by directed evolution for application in industrial biotechnology, BP.DR - associated scholarships

Abstract

In the 70s, Brazil started a program to substitute gasoline by ethanol in order to decrease dependence from politically and economically variable periods. The plant species chosen was sugarcane and as a consequence, agricultural and technological studies were greatly intensified, leading Brazil to a very favorable position in terms of energy security. Nowadays, Brazil has more than 80% of its cars running with bioethanol and even airplane engines are now being developed. With the increasing political instability in the Middle East, since 2001, the USA has also decided to direct its energy policy towards the use of biofuels. This is now being followed by Europe and Japan and it is likely to be followed by several other countries in the world. This imposes an enormous pressure on the production of crops that can supply bioethanol. Brazilian sugarcane is probably the most efficient extant bioethanol producing system. However, only part of the biomass produced is used for bioenergy production, 1/3 of the plant being used for sucrose production, 1/3 is bagasse, which is burnt for production of electricity and the last third is left in the field and latter on decomposed by microorganisms. Therefore, in order to supply wider needs, a significant increase in production of ethanol is possible if we can provide the basic knowledge necessary for development of technologies that will be capable to obtain energy from the polysaccharides of the cell wall, which makes 70% of the biomass burnt inefficiently and left in the field. The availability of such processes within the distillery and the higher marketing value of liquid fuel provide additional economical advantages to its conversion instead of simply burning bagasse. Although the chemical hydrolysis of biomass is a consolidated methodology under laboratory conditions, its large-scale application is not economically viable, yet. The necessary use of acids reduces the life-time of equipments, produces toxic wastes and produces non-fermentable sugars, Increasing the costs of the products. One alternative is the enzymatic hydrolysis of the cell wall. Such a process requires the use of a complex machinery of specific enzymes that are produced either by the plant itself or by microorganism able to degrade plant cell walls. On the other hand, relatively little is known about the structure and architecture of the cell wall. One of the goals of the IN CT do Bioetanol (Figure 1) is understand the fine structure of the principal hemicelluloses of sugarcane and other possible sources of biomass. We intend to find patterns of gene expression that could be useful to find ways to induce the plants to degrade their own wall and become prepared for subsequent hydrolysis. In parallel, we intend to prospect microorganisms, enzymes and genes both in microorganisms and sugarcane, that are capable to efficiently hydrolyze the walls. Such enzymes will be designed to have the highest possible performance to degrade plant cell walls, especially the walls of sugarcane. A group of researchers screen existent varieties of sugarcane to find gene markers that could guide the groups to quickly identify plant materials that would be more suitable for use in industrial processes. From the latter viewpoint, our group intends to perform tests of mechanical preparation of sugarcane for further acid and/or enzymatic hydrolysis. With this data in hands, we expect to be able to provide the fundamental knowledge of biotechnology that is necessary for scaling up studies and further increase in efficient of bioethanol production in Brazil. (AU)

Articles published in Agência FAPESP Newsletter about the research grant
Africa is crucial for Brazil to exercise leadership in biofuels, study concludes 
Researchers uncover defense code for sugarcane 
Book presents the main scientific advances in bioenergy 
The success of sugarcane is the fruit of pioneering science and technology, study concludes 
Articles published in Pesquisa FAPESP Maganize about the research grant:
Between sugars and genes 

Scientific publications (27)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BARRETO, FERNANDA ZATTI; BACHEGA FEIJO ROSA, JOAO RICARDO; ALMEIDA BALSALOBRE, THIAGO WILLIAN; PASTINA, MARIA MARTA; SILVA, RENATO RODRIGUES; HOFFMANN, HERMANN PAULO; DE SOUZA, ANETE PEREIRA; FRANCO GARCIA, ANTONIO AUGUSTO; CARNEIRO, MONALISA SAMPAIO. A genome-wide association study identified loci for yield component traits in sugarcane (Saccharum spp.). PLoS One, v. 14, n. 7 JUL 18 2019. Web of Science Citations: 1.
JARDIM, VINICIUS CARVALHO; SANTOS, SUZANA DE SIQUEIRA; FUJITA, ANDRE; BUCKERIDGE, MARCOS SILVEIRA. BioNetStat: A Tool for Biological Networks Differential Analysis. FRONTIERS IN GENETICS, v. 10, JUN 21 2019. Web of Science Citations: 0.
GODOY-VEIGA, MILENA; SLOTTA, FRANZISKA; ALECIO, PAULA CHRISTIANI; CECCANTINI, GREGORIO; BUCKERIDGE, MARCOS SILVEIRA; LOCOSSELLI, GIULIANO MASELLI. Improved tree-ring visualization using autofluorescence. DENDROCHRONOLOGIA, v. 55, p. 33-42, JUN 2019. Web of Science Citations: 0.
LOCOSSELLI, GIULIANO MASELLI; DE CAMARGO, EVELYN PEREIRA; LOPES MOREIRA, TIANA CARLA; TODESCO, ENZO; ANDRADE, MARIA DE FATIMA; SALDIVA DE ANDRE, CARMEN DIVA; DE ANDRE, PAULO AFONSO; SINGER, JULIO M.; FERREIRA, LUCIANA SCHWANDNER; NASCIMENTO SALDIVA, PAULO HILARIO; BUCKERIDGE, MARCOS SILVEIRA. The role of air pollution and climate on the growth of urban trees. Science of The Total Environment, v. 666, p. 652-661, MAY 20 2019. Web of Science Citations: 2.
TAVARES, EVELINE Q. P.; DE SOUZA, AMANDA P.; ROMIM, GRAYCE H.; GRANDIS, ADRIANA; PLASENCIA, ANNA; GAIARSA, JONAS W.; GRIMA-PETTENATI, JACQUELINE; DE SETTA, NATHALIA; VAN SLUYS, MARIE-ANNE; BUCKERIDGE, MARCOS S. The control of endopolygalacturonase expression by the sugarcane RAV transcription factor during aerenchyma formation. Journal of Experimental Botany, v. 70, n. 2, p. 497-506, JAN 15 2019. Web of Science Citations: 0.
VIEIRA MANECHINI, JOAO RICARDO; DA COSTA, JULIANA BORGES; PEREIRA, BRUNA TURCATTO; CARLINI-GARCIA, LUCIANA APARECIDA; XAVIER, MAURO ALEXANDRE; DE ANDRADE LANDELL, MARCOS GUIMARAES; PINTO, LUCIANA ROSSINI. Unraveling the genetic structure of Brazilian commercial sugarcane cultivars through microsatellite markers. PLoS One, v. 13, n. 4 APR 23 2018. Web of Science Citations: 3.
BUCKERIDGE, MARCOS S. The evolution of the glycomic codes of extracellular matrices. BIOSYSTEMS, v. 164, n. SI, p. 112-120, FEB 2018. Web of Science Citations: 3.
DE SOUZA, AMANDA P.; GRANDIS, ADRIANA; ARENQUE-MUSA, BRUNA C.; BUCKERIDGE, MARCOS S. Diurnal variation in gas exchange and nonstructural carbohydrates throughout sugarcane development. FUNCTIONAL PLANT BIOLOGY, v. 45, n. 8, p. 865-876, 2018. Web of Science Citations: 2.
TAVARES, E. Q. P.; GRANDIS, A.; LEMBKE, C. G.; SOUZA, G. M.; PURGATTO, E.; DE SOUZA, A. P.; BUCKERIDGE, M. S. Roles of auxin and ethylene in aerenchyma formation in sugarcane roots. PLANT SIGNALING & BEHAVIOR, v. 13, n. 3 2018. Web of Science Citations: 3.
LEITE, D. C. C.; GRANDIS, A.; TAVARES, E. Q. P.; PIOVEZANI, A. R.; PATTATHIL, S.; AVCI, U.; ROSSINI, A.; CAMBLER, A.; DE SOUZA, A. P.; HAHN, M. G.; BUCKERIDGE, M. S. Cell wall changes during the formation of aerenchyma in sugarcane roots. ANNALS OF BOTANY, v. 120, n. 5, SI, p. 693-708, NOV 2017. Web of Science Citations: 6.
ALMEIDA BALSALOBRE, THIAGO WILLIAN; PEREIRA, GUILHERME DA SILVA; ALVES MARGARIDO, GABRIEL RODRIGUES; GAZAFFI, RODRIGO; BARRETO, FERNANDA ZATTI; ANONI, CARINA OLIVEIRA; CARDOSO-SILVA, CLAUDIO BENICIO; COSTA, ESTELA ARAUJO; MANCINI, MELINA CRISTINA; HOFFMANN, HERMANN PAULO; DE SOUZA, ANETE PEREIRA; FRANCO GARCIA, ANTONIO AUGUSTO; CARNEIRO, MONALISA SAMPAIO. GBS-based single dosage markers for linkage and QTL mapping allow gene mining for yield-related traits in sugarcane. BMC Genomics, v. 18, JAN 11 2017. Web of Science Citations: 19.
LATARULLO, MARIANA B. G.; TAVARES, EVELINE Q. P.; MALDONADO, GABRIEL P.; LEITE, DEBORA C. C.; BUCKERIDGE, MARCOS S. Pectins, Endopolygalacturonases, and Bioenergy. FRONTIERS IN PLANT SCIENCE, v. 7, SEP 20 2016. Web of Science Citations: 15.
COSTA, E. A.; ANONI, C. O.; MANCINI, M. C.; SANTOS, F. R. C.; MARCONI, T. G.; GAZAFFI, R.; PASTINA, M. M.; PERECIN, D.; MOLLINARI, M.; XAVIER, M. A.; PINTO, L. R.; SOUZA, A. P.; GARCIA, A. A. F. QTL mapping including codominant SNP markers with ploidy level information in a sugarcane progeny. EUPHYTICA, v. 211, n. 1, p. 1-16, SEP 2016. Web of Science Citations: 7.
AFIONIS, STAVROS; STRINGER, LINDSAY C.; FAVRETTO, NICOLA; TOMEI, JULIA; BUCKERIDGE, MARCOS S. Unpacking Brazil's Leadership in the Global Biofuels Arena: Brazilian Ethanol Diplomacy in Africa. GLOBAL ENVIRONMENTAL POLITICS, v. 16, n. 3, p. 127-150, AUG 2016. Web of Science Citations: 2.
SOUZA, THAIS P.; DIAS, RENATA O.; CASTELHANO, ELAINE C.; BRANDAO, MARCELO M.; MOURA, DANIEL S.; SILVA-FILHO, MARCIO C. Comparative analysis of expression profiling of the trypsin and chymotrypsin genes from Lepidoptera species with different levels of sensitivity to soybean peptidase inhibitors. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 196, p. 67-73, JUN-JUL 2016. Web of Science Citations: 4.
MELLONI, MARIA NATALIA G.; MELLONI, MARIA LETICIA G.; NEUBER, ANA CAROLINE; PERECIN, DILERMANDO; LANDELL, MARCOS G. DE ANDRADE; PINTO, LUCIANA R. Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane. SUGAR TECH, v. 18, n. 2, p. 222-228, APR 2016. Web of Science Citations: 1.
BUCKERIDGE, MARCOS S.; DE SOUZA, AMANDA P. Breaking the ``Glycomic Code{''} of Cell Wall Polysaccharides May Improve Second-Generation Bioenergy Production from Biomass. BioEnergy Research, v. 7, n. 4, p. 1065-1073, DEC 2014. Web of Science Citations: 0.
RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; JORGE, JOAO A.; PRADE, ROLF A.; BUCKERIDGE, MARCOS S.; POLIZELI, MARIA DE LOURDES T. M. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, JUL 29 2014. Web of Science Citations: 28.
FRANCO, FLAVIA P.; SANTIAGO, ADELITA C.; HENRIQUE-SILVA, FLAVIO; DE CASTRO, PATRICIA ALVES; GOLDMAN, GUSTAVO H.; MOURA, DANIEL S.; SILVA-FILHO, MARCIO C. The Sugarcane Defense Protein SUGARWIN2 Causes Cell Death in Colletotrichum falcatum but Not in Non-Pathogenic Fungi. PLoS One, v. 9, n. 3 MAR 7 2014. Web of Science Citations: 7.
VALENCIA, ESTELA Y.; CHAMBERGO, FELIPE S. Mini-review: Brazilian fungi diversity for biomass degradation. Fungal Genetics and Biology, v. 60, n. SI, p. 9-18, NOV 2013. Web of Science Citations: 10.
FONSECA-MALDONADO, RAQUEL; VIEIRA, DAVI SERRADELLA; ALPONTI, JULIANA SANCHEZ; BONNEIL, ERIC; THIBAULT, PIERRE; WARD, RICHARD JOHN. Engineering the Pattern of Protein Glycosylation Modulates the Thermostability of a GH11 Xylanase. Journal of Biological Chemistry, v. 288, n. 35, p. 25522-25534, AUG 30 2013. Web of Science Citations: 32.
DE SOUZA, AMANDA P.; LEITE, DEBORA C. C.; PATTATHIL, SIVAKUMAR; HAHN, MICHAEL G.; BUCKERIDGE, MARCOS S. Composition and Structure of Sugarcane Cell Wall Polysaccharides: Implications for Second-Generation Bioethanol Production. BioEnergy Research, v. 6, n. 2, p. 564-579, JUN 2013. Web of Science Citations: 12.
GENTILE, AGUSTINA; FERREIRA, THAIS H.; MATTOS, RAPHAEL S.; DIAS, LARA I.; HOSHINO, ANDREA A.; CARNEIRO, MONALISA S.; SOUZA, GLAUCIA M.; CALSA, JR., TERCILIO; NOGUEIRA, REJANE M.; ENDRES, LAURICIO; MENOSSI, MARCELO. Effects of drought on the microtranscriptome of field-grown sugarcane plants. PLANTA, v. 237, n. 3, p. 783-798, MAR 2013. Web of Science Citations: 28.
BEGCY, KEVIN; MARIANO, EDUARDO D.; GENTILE, AGUSTINA; LEMBKE, CAROLINA G.; ZINGARETTI, SONIA MARLI; SOUZA, GLAUCIA M.; MENOSSI, MARCELO. A Novel Stress-Induced Sugarcane Gene Confers Tolerance to Drought, Salt and Oxidative Stress in Transgenic Tobacco Plants. PLoS One, v. 7, n. 9 SEP 11 2012. Web of Science Citations: 22.
MEDEIROS, ANE H.; FRANCO, FLAVIA P.; MATOS, JULIANA L.; DE CASTRO, PATRICIA A.; SANTOS-SILVA, LUDIER K.; HENRIQUE-SILVA, FLAVIO; GOLDMAN, GUSTAVO H.; MOURA, DANIEL S.; SILVA-FILHO, MARCIO C. Sugarwin: A Sugarcane Insect-Induced Gene with Antipathogenic Activity. MOLECULAR PLANT-MICROBE INTERACTIONS, v. 25, n. 5, p. 613-624, MAY 2012. Web of Science Citations: 10.
PASTINA, M. M.; MALOSETTI, M.; GAZAFFI, R.; MOLLINARI, M.; MARGARIDO, G. R. A.; OLIVEIRA, K. M.; PINTO, L. R.; SOUZA, A. P.; VAN EEUWIJK, F. A.; GARCIA, A. A. F. A mixed model QTL analysis for sugarcane multiple-harvest-location trial data. THEORETICAL AND APPLIED GENETICS, v. 124, n. 5, p. 835-849, MAR 2012. Web of Science Citations: 42.
FURTADO, GILVAN PESSOA; RIBEIRO, LUCAS FERREIRA; SANTOS, CAMILA RAMOS; TONOLI, CELISA CALDANA; DE SOUZA, ANGELICA RODRIGUES; OLIVEIRA, RENATA ROCHA; MURAKAMI, MARIO TYAGO; WARD, RICHARD JOHN. Biochemical and structural characterization of a beta-1,3-1,4-glucanase from Bacillus subtilis 168. Process Biochemistry, v. 46, n. 5, p. 1202-1206, MAY 2011. Web of Science Citations: 29.

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