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Development of an enzymatic cocktail for application in biorefinery

Grant number: 10/10184-3
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2010
Effective date (End): February 28, 2014
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Maria de Lourdes Teixeira de Moraes Polizeli
Grantee:Liliane Fraga Costa Ribeiro
Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:08/57908-6 - National Institute of Science and Technology of Bioethanol, AP.BIOEN.TEM


The use of ethanol as fuel has advantages as it is an inexhaustible energy source and because ethanol combustion lead to reduced CO2 emissions compared to fossil fuels. The production of bioethanol mainly uses molasses from sugar cane, discarding the pulp. The pulp, or bagasse, is a residue rich in cellulose and hemicellulose, that is why it can also be used for ethanol production, the so-called second generation ethanol. Thus, this work aims to develop an enzymatic cocktail containing cellulolytic and xylanolytic enzymes that can be used on bagasse pre-treated, releasing sugars that can be used by yeasts, such as Saccharomyces, for the production of bioethanol. The use of an enzymatic mix, using enzymes that will be cloned and expressed in filamentous fungi, and the fact they can be reused, because it will be immobilized in chemical supports, is an innovation in the research of our group and therefore has much to contribute to scientific growth in our laboratory. This project is inserted into the theme promoted by FAPESP, National Institute of Biotechnology for Ethanol, under the coordination of Professor Dr. Marcos Silveira Buckeridge, in partnership with CNPq - Announcement of the National Science, Technology and Innovation.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIBEIRO, LILIANE F. C.; DE LUCAS, ROSYMAR C.; VITCOSQUE, GABRIELA L.; RIBEIRO, LUCAS F.; WARD, RICHARD J.; RUBIO, MARCELO V.; DAMASIO, ANDRE R. L.; SQUINA, FABIO M.; GREGORY, REBECCA C.; WALTON, PAUL H.; et al. A novel thermostable xylanase GH10 from Malbranchea pulchella expressed in Aspergillus nidulans with potential applications in biotechnology. BIOTECHNOLOGY FOR BIOFUELS, v. 7, . (10/18850-2, 12/12859-3, 13/18910-3, 10/07133-8, 10/10184-3, 12/02017-5, 08/57908-6)
VITCOSQUE, GABRIELA LEAL; RIBEIRO, LILIANE FRAGA COSTA; DE LUCAS, ROSYMAR COUTINHO; DA SILVA, TONY MARCIO; RIBEIRO, LUCAS FERREIRA; DE LIMA DAMASIO, ANDRE RICARDO; FARINAS, CRISTIANE SANCHEZ; LOPES GONCALVES, ALINE ZORZETTO; SEGATO, FERNANDO; BUCKERIDGE, MARCOS SILVEIRA; et al. The functional properties of a xyloglucanase (GH12) of Aspergillus terreus expressed in Aspergillus nidulans may increase performance of biomass degradation. Applied Microbiology and Biotechnology, v. 100, n. 21, p. 9133-9144, . (10/10184-3, 14/18714-2, 12/20549-4)
RIBEIRO, LUCAS FERREIRA; TULLMAN, JENNIFER; NICHOLES, NATHAN; BERGAMACHI SILVA, SERGIO RUSCHI; VIEIRA, DAVI SERRADELLA; OSTERMEIER, MARC; WARD, RICHARD JOHN. A xylose-stimulated xylanase-xylose binding protein chimera created by random nonhomologous recombination. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . (10/18850-2, 10/10184-3, 10/07133-8)

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