epatic enzymes and immunoinflammatory response to Bio-C Temp bioceramic intracanal medication implanted into the subcutaneous tissue of rat

Autor(es)

Lopes, Camila Soares ^[1] ; Delfino, Mateus Machado ^[1] ; Tanomaru-Filho, Mario ^[1] ; Sasso-Cerri, Estela ^[2] ; Guerreiro-Tanomaru, Juliane Maria ^[1] ; Cerri, Paulo Sergio ^[2]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Dent Sch, Dept Restorat Dent, Araraquara, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Dent Sch, Dept Morphol Genet Orthodont & Pediat Dent, Lab Histol & Embryol, Araraquara, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 12, n. 1 FEB 18 2022.
• Citações Web of Science: 0

Resumo

Our purpose was to evaluate the biocompatibility and hepatotoxicity of a new bioceramic intracanal medicament, Bio-C Temp (BIO). The biological properties of BIO were compared with calcium hydroxide-based intracanal medicament (Calen; CAL), used as gold pattern. Polyethylene tubes filled with BIO or CAL, and empty tubes (control group, CG) were implanted into subcutaneous tissue of rats. After 7, 15, 30 and 60 days, the samples were embedded in paraffin for morphological, quantitative and immunohistochemistry analyses. At 7 and 60 days, blood samples were collected for analysis of serum glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) levels. The data were submitted to two-way ANOVA and Tukey's test (p <= 0.05). No significant difference was detected in serum GOT and GPT levels among BIO, CAL and CG specimens. In all periods, BIO specimens exhibited lower number of inflammatory cells and immunoexpression of IL-6, a pro-inflammatory cytokine, than CAL specimens. The reduction of these parameters was accompanied by significant increase in the collagen content and in the immunoexpression of IL-10, a cytokine involved in the tissue repair, over time. Our findings indicate that Bio-C Temp is biocompatible and had no hepatotoxicity effect. (AU)

• Processo FAPESP: 18/16848-2 - Avaliação das propriedades biológicas, físicas e químicas da medicação intracanal à base de biocerâmico BIO-C temp
• Beneficiário: Camila Soares Lopes
• Modalidade de apoio: Bolsas no Brasil - Doutorado