Infection by Moniliophthora perniciosa reprograms tomato Micro-Tom physiology, establishes a sink, and increases secondary cell wall synthesis

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Paschoal, Daniele ; Costa, Juliana L. ; da Silva, Eder M. ; da Silva, Fabia B. ; Capelin, Diogo ; Ometto, Vitor ; Aricetti, Juliana A. ; Carvalho, Gabriel G. ; Pimpinato, Rodrigo F. ; de Oliveira, Ricardo F. ; Carrera, Esther ; Lopez-Diaz, Isabel ; Rossi, Monica L. ; Tornisielo, Valdemar ; Caldana, Camila ; Riano-Pachon, Diego M. ; Cesarino, Igor ; Teixeira, Paulo J. P. L. ; Figueira, Antonio

• Tipo de documento: Artigo Científico
• Fonte: Journal of Experimental Botany; v. 73, n. 11, p. 20-pg., 2022-02-21.

Resumo

Witches' broom disease of cacao is caused by the pathogenic fungus Moniliophthora perniciosa. By using tomato (Solanum lycopersicum) cultivar Micro-Tom (MT) as a model system, we investigated the physiological and metabolic consequences of M. perniciosa infection to determine whether symptoms result from sink establishment during infection. Infection of MT by M. perniciosa caused reductions in root biomass and fruit yield, a decrease in leaf gas exchange, and down-regulation of photosynthesis-related genes. The total leaf area and water potential decreased, while ABA levels, water conductance/conductivity, and ABA-related gene expression increased. Genes related to sugar metabolism and those involved in secondary cell wall deposition were up-regulated upon infection, and the concentrations of sugars, fumarate, and amino acids increased. C-14-glucose was mobilized towards infected MT stems, but not in inoculated stems of the MT line overexpressing CYTOKININ OXIDASE-2 (35S::AtCKX2), suggesting a role for cytokinin in establishing a sugar sink. The up-regulation of genes involved in cell wall deposition and phenylpropanoid metabolism in infected MT, but not in 35S::AtCKX2 plants, suggests establishment of a cytokinin-mediated sink that promotes tissue overgrowth with an increase in lignin. Possibly, M. perniciosa could benefit from the accumulation of secondary cell walls during its saprotrophic phase of infection. Infection by Moniliophthora perniciosaof tomato Micro-Tom induces a cytokinin-mediated nutrient sink at the stem and leads to vascular overgrowth and accumulation of secondary cell wall. (AU)

• Processo FAPESP: 13/04309-6 - Análise funcional de genes de sinalização e defesa do cacaueiro na interação Moniliophthora perniciosa x Solanum lycopersicum
• Beneficiário: Juliana Leles Costa
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/17000-4 - Análise da interação Moniliophthora perniciosa x tomateiro 'Micro-Tom': resistência do tipo 'não hospedeiro', efeitos no desenvolvimento e papel dos fitohormônios na patogênese
• Beneficiário: Eder Marques da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 16/10498-4 - Investigação das estratégias de adaptação ao estilo de vida patogênico de fungos do gênero Moniliophthora em diferentes níveis de organização biológica: espécies, biótipos e linhagens geográficas
• Beneficiário: Antonio Vargas de Oliveira Figueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/10524-5 - Caracterização do perfil transcricional na interação Moniliophthora perniciosa x Solanum lycopersicum
• Beneficiário: Juliana Leles Costa
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 18/18711-4 - Análise funcional de genes potencialmente associados à resistência ao basidiomiceto Moniliophthora perniciosa em tomateiro (Solanum lycopersicum) cv. 'Micro-Tom'
• Beneficiário: Daniele Paschoal
• Modalidade de apoio: Bolsas no Brasil - Doutorado