Convergent and lineage-specific genomic differences in limb regulatory elements in limbless reptile lineages

Autor(es)

Roscito, Juliana Gusson ; Sameith, Katrin ; Kirilenko, Bogdan Mikhailovich ; Hecker, Nikolai ; Winkler, Sylke ; Dahl, Andreas ; Rodrigues, Miguel Trefaut ; Hiller, Michael

• Tipo de documento: Artigo Científico
• Fonte: CELL REPORTS; v. 38, n. 3, p. 24-pg., 2022-01-18.

Resumo

Loss of limbs evolved many times in squamate reptiles. Here we investigated the genomic basis of convergent limb loss in reptiles. We sequenced the genomes of a closely related pair of limbless-limbed gymnophthalmid lizards and performed a comparative genomic analysis including five snakes and the limbless glass lizard. Our analysis of these three independent limbless lineages revealed that signatures of shared sequence or transcription factor binding site divergence in individual limb regulatory elements are generally rare. Instead, shared divergence occurs more often at the level of signaling pathways, involving different regulatory elements associated with the same limb genes (such as Hand2 or Hox) and/or patterning mechanisms (such as Shh signaling). Interestingly, although snakes are known to have mutations in the Shh ZRS limb enhancer, this enhancer lacks relevant mutations in limbless lizards. Thus, different mechanisms could contribute to limb loss, and there are likely multiple evolutionary paths to limblessness in reptiles. (AU)

• Processo FAPESP: 12/01319-8 - Evolução dos mecanismos de desenvolvimento envolvidos com a redução dos membros em Squamata
• Beneficiário: Juliana Gusson Roscito
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado