Muscle Stem Cell Function Is Impaired in beta 2-Adrenoceptor Knockout Mice

Autor(es)

Koike, Tatiana E. ; Fuziwara, Cesar S. ; Brum, Patricia C. ; Kimura, Edna T. ; Rando, Thomas A. ; Miyabara, Elen H.

• Tipo de documento: Artigo Científico
• Fonte: STEM CELL REVIEWS AND REPORTS; v. N/A, p. 13-pg., 2022-03-04.

Resumo

Knockout (ko) mice for the beta 2 adrenoceptor (Adr beta 2) have impaired skeletal muscle regeneration, suggesting that this receptor is important for muscle stem cell (satellite cell) function. Here, we investigated the role of Adr beta 2 in the function of satellite cells from beta 2ko mice in the context of muscle regeneration, through in vivo and in vitro experiments. Immunohistochemical analysis showed a significant reduction in the number of self-renewed Pax7(+) satellite cells, proliferating Pax7(+)/MyoD(+) myogenic precursor cells, and regenerating eMHC(+) myofibers in regenerating muscle of beta 2ko mice at 30, 3, and 10 days post-injury, respectively. Quiescent satellite cells were isolated by fluorescence-activated cell sorting, and cell cycle entry was assessed by EdU incorporation. The results demonstrated a lower number of proliferating Pax7(+)/EdU(+) satellite cells from beta 2ko mice. There was an increase in the gene expression of the cell cycle inhibitor Cdkn1a and Notch pathway components and the activation of Notch signaling in proliferating myoblasts from beta 2ko mice. There was a decrease in the number of myogenin-positive nuclei in myofibers maintained in differentiation media, and a lower fusion index in differentiating myoblasts from beta 2ko mice. Furthermore, the gene expression of Wnt/beta-catenin signaling components, the expression of nuclear beta-catenin and the activation of Wnt/beta-catenin signaling decreased in differentiating myoblasts from beta 2ko mice. These results indicate that Adr beta 2 plays a crucial role in satellite cell self-renewal, as well as in myoblast proliferation and differentiation by regulating Notch and Wnt/beta-catenin signaling, respectively. (AU)

• Processo FAPESP: 17/09069-4 - Influência do adrenoceptor beta 2 na função das células satélite
• Beneficiário: Tatiana Emy Koike
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/23391-8 - Resposta regenerativa da musculatura esquelética de camundongos com síndrome de Marfan
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/24946-4 - Reabilitação do músculo esquelético de camundongos com síndrome de Marfan: efeito do exercício excêntrico
• Beneficiário: Elen Haruka Miyabara
• Modalidade de apoio: Auxílio à Pesquisa - Regular