Autor(es)

de Camargo, Marcela Rodrigues ; Frazon, Talita Fonseca ; Inacio, Kelly Karina ; Smiderle, Fhernanda Ribeiro ; Amor, Nadia Ghinelli ; Dionisio, Thiago Jose ; Santos, Carlos Ferreira ; Rodini, Camila Oliveira ; Lara, Vanessa Soares

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 286, p. 15-pg., 2022-03-25.

Resumo

Ethnopharmacological relevance: The polysaccharides of the millenary mushroom Ganoderma lucidum (GL) have been shown for decades to present anti-tumor activities, but few studies evaluated its importance on cancer stem cells and EMT process. Cancer stem cells (CSC) drive the development of carcinoma and are also involved in cancer treatment failure, being a good target for treatment success. Also, the process of epithelial-mesenchymal transition (EMT) is involved in metastasis and cancer relapse. Besides that, the increasing incidence worldwide of oral squamous cell carcinoma (OSCC) became a public health issue with a high rate of metastasis and poor quality of life for patients during and after treatment.Aim of the study: to evaluate G. lucidum polysaccharides (GLPS) in vitro effects on OSCC, focusing on hallmarks associated with tumorigenesis using the SCC-9, a squamous cells carcinoma lineage from the tongue.Materials and methods: SCC-9 cells were treated in vitro for 72h with different GLPS concentrations. The controls cells were maintained with culture media only and cisplatin was used as treatment control. After the treatment period, the cells were evaluated.Results: GLPS treatment changed cell morphology and granularity, delayed migration, decreased colony, and impaired sphere formation, thereby leading to a non-invasive and less proliferative behavior of tumoral cells. Additionally, GLPS downregulated CSC, EMT, and drug sensitivity (ABC) markers.Conclusions: These results show that the natural product GLPS has the potential to be an important ally for tongue squamous cell carcinoma treatment, bringing the millenary compound to modern therapy, providing a basis for future studies and the improvement of life quality for OSCC patients. (AU)

• Processo FAPESP: 13/07245-9 - Investigação do papel das células-tronco de câncer e do microambiente no processo de transição epitélio-mesenquimal, invasão e metástase do carcinoma epidermóide de boca
• Beneficiário: Camila de Oliveira Rodini Pegoraro
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 17/01212-2 - Avaliação in vitro da ação antitumoral de polissacarídeos de Ganoderma Lucidum associados a quimioterapia metronômica por 5-FU sobre células-tronco de carcinoma espinocelular de cabeça e pescoço.
• Beneficiário: Marcela Rodrigues de Camargo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 18/04203-7 - Estudo da ação anti-tumoral de polissacarídeos de Ganoderma lucidum sobre células tronco de carcinoma espinocelular de língua
• Beneficiário: Talita Fonseca Frazon
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 15/12894-1 - EMU concedido no processo 2013/07245-9: citômetro de fluxo BD FACSAria com BD FACSAria ACDU e acessórios
• Beneficiário: Camila de Oliveira Rodini Pegoraro
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários