Biosynthesis of Guanitoxin Enables Global Environmental Detection in Freshwater Cyanobacteria

Autor(es):Mostrar menos -

Lima, Stella T. ; Fallon, Timothy R. ; Cordoza, Jennifer L. ; Chekan, Jonathan R. ; Delbaje, Endrews ; Hopiavuori, Austin R. ; Alvarenga, Danillo O. ; Wood, Steffaney M. ; Luhavaya, Hanna ; Baumgartner, Jackson T. ; Dorr, Felipe A. ; Etchegaray, Augusto ; Pinto, Ernani ; McKinnie, Shaun M. K. ; Fiore, Marli F. ; Moore, Bradley S.

• Tipo de documento: Artigo Científico
• Fonte: Journal of the American Chemical Society; v. N/A, p. 8-pg., 2022-05-18.

Resumo

Harmful cyanobacterial blooms (cyanoHABs) cause recurrent toxic events in global watersheds. Although public health agencies monitor the causal toxins of most cyanoHABs and scientists in the field continue developing precise detection and prediction tools, the potent anticholinesterase neurotoxin, guanitoxin, is not presently environ-mentally monitored. This is largely due to its incompatibility with widely employed analytical methods and instability in the environment, despite guanitoxin being among the most lethal cyanotoxins. Here, we describe the guanitoxin biosynthesis gene cluster and its rigorously characterized nine-step metabolic pathway from L-arginine in the cyanobacterium Sphaerospermopsis torques-reginae ITEP-024. Through environmental sequencing data sets, guanitoxin (gnt) biosynthetic genes are repeatedly detected and expressed in municipal freshwater bodies that have undergone past toxic events. Knowledge of the genetic basis of guanitoxin biosynthesis now allows for environmental, biosynthetic gene monitoring to establish the global scope of this neurotoxic organophosphate. (AU)

• Processo FAPESP: 11/08092-6 - Sequenciamento do genoma da cianobactéria Nostoc sp. CENA67 e anotação de genes do metabolismo secundário
• Beneficiário: Danillo Oliveira de Alvarenga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/50425-8 - Biodiversity of cyanobacteria and their bioactive compounds from under-explored Brazilian habitats
• Beneficiário: Marli de Fátima Fiore
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/06869-0 - Expressão heteróloga, purificação e caracterização de enzimas envolvidas na via biossintética da neurotoxina cianobacteriana anatoxina-a(s)
• Beneficiário: Stella de Lima Camargo
• Modalidade de apoio: Bolsas no Brasil - Doutorado