Effect of thermotherapy, Leifsonia xyli subsp. xyli titres, sugarcane genotype and diagnostic techniques on ratoon stunt control in Brazil

Autor(es)

Andreato, Caroline ; Gazaffi, Rodrigo ; Aguiar Oliveira, Maysa Mariano ; Aranha Camargo, Luis Eduardo ; Urashima, Alfredo Seiiti

• Tipo de documento: Artigo Científico
• Fonte: Journal of Applied Microbiology; v. N/A, p. 12-pg., 2022-06-29.

Resumo

Aims To examine the interaction of diagnostic techniques, initial titres of Leifsonia xyli subsp. xyli (Lxx), sugarcane genotype and thermotherapy on ratoon stunt (RSD) control. Methods and Results Single buds of RB867515, RB92579 and RB966928 were submitted to 50 degrees C/2 h or 52 degrees C/30 min under factorial block design and five replications; results were checked 9 months later by serological (DBI) and molecular (PCR) techniques. A 10,000 bootstrapping simulations were performed to infer the best plot size based on the experimental coefficient of variation. Analysis of variance showed significance only on initial Lxx titres and RSD control. Despite the absence of significance in the overall analysis, minor differences in control success with different methods and cultivars are predicted to have a major epidemiological impact on RSD, considering successive harvests and vegetative increase. According to an epidemiological interpretation, the 50 degrees C/2 h treatment was more effective, cultivar RB966928 was the most susceptible and the PCR-based method was the most sensitive for pathogen detection. The minimum required plants per plot was 15, indicating high precision of our experiment Conclusions Data interpretation considered both the statistical analysis and the epidemiology aspect of RSD in order to improve RSD management. The Brazilian sugarcane industry will benefit from this approach since it is not using it. Significance and Impact of the Study This is the first study that examined multiple factors that affect RSD control. Our work pinpointed the importance of the thermotherapy, its best combination as well as the diagnostic test. Also, the effect of the cultivar to respond to management strategies. Because the epidemiological aspect of RSD was taken into consideration, results of our work can have an impact on RSD control in the field. (AU)

• Processo FAPESP: 19/11424-2 - Efeito da termoterapia na produtividade de cana-de-açúcar, no escape de Leifsonia xyli subsp. xyli em função de sua concentração e detecção precoce pelos diferentes métodos diagnósticos
• Beneficiário: Maysa Mariano Aguiar de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 17/18469-6 - Manejo do raquitismo-das-soqueiras: efeito da termoterapia atual na produtividade de cana-de-açúcar e controle de Leifsonia xyli subsp. xyli
• Beneficiário: Alfredo Seiiti Urashima
• Modalidade de apoio: Auxílio à Pesquisa - Regular