Photobiomodulation and photodynamic therapy applied after electrocauterization for skin healing optimization in rats

Autor(es)

Manoel, Cecilia do Amparo ; Mariano, Samara de Sousa ; Ramos, Ericsson da Silva ; Paolillo, Fernanda Rossi ; de Aro, Andrea Aparecida ; Mendes, Carolini ; Venturini, Ligia Milanez ; Lock Silveira, Paulo Cesar ; Bagnato, Vanderlei Salvador ; Moretti de Andrade, Thiago Antonio

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 15, n. 5, p. 16-pg., 2022-02-07.

Resumo

Photobiomodulation-PBM and Photodynamic Therapy-PDT have been used to induce healing. However, the effects of these therapies on skin-lesions induced by electrocautery are unknown, aiming at more favorable clinical and esthetic results. Electrocauterization was done in 78-female Wistar-rats using a system that includes an electrocautery and red-LED. The groups were: No injury, Injury, Injury + ALA (topical 5-aminolevulinic acid application), Injury + LED and Injury + ALA + LED (topical ALA application followed by photoactivation with LED). After 2nd, 7th and 14th days post-injury, immuno-histomorphometric analyses (inflammatory infiltrate, blood vessels, fibroblasts, eschar/epidermal thickness, IL-10 and VEGF) and biochemical assays of MPO (neutrophil), NAG (macrophage), nitrite, DCF (H2O2), carbonyl (membrane's damage), sulfhydryl (membrane's integrity), SOD, GSH, hydroxyproline and re-epithelialization area were performed. The Injury + LED and Injury + ALA + LED groups controlled inflammation and oxidative stress, favoring angiogenesis, fibroblasts proliferation and collagen formation. Therefore, the PBM or PDT was effective in tissue formation with thinner eschar and epidermis, resulting in less scarring after electrocauterization. (AU)

• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs