A Brazilian Inter-Hospital Candidemia Outbreak Caused by Fluconazole-Resistant Candida parapsilosis in the COVID-19 Era

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Thomaz, Danilo Y. ; Del Negro, Gilda M. B. ; Ribeiro, Leidiane B. ; da Silva, Mirian ; Carvalho, Gabrielle O. M. H. ; Camargo, Carlos H. ; de Almeida, Joao N. ; Motta, Adriana L. ; Siciliano, Rinaldo F. ; Sejas, Odeli N. E. ; Rossi, Flavia ; Abdala, Edson ; Strabelli, Tania M. V. ; Benard, Gil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FUNGI; v. 8, n. 2, p. 8-pg., 2022-02-01.

Resumo

Horizontal transmission of fluconazole-resistant Candida parapsilosis (FRCP) through healthcare workers' hands has contributed to the occurrence of candidemia outbreaks worldwide. Since the first COVID-19 case in Brazil was detected in early 2020, hospitals have reinforced hand hygiene and disinfection practices to minimize SARS-CoV-2 contamination. However, a Brazilian cardiology center, which shares ICU patients with a cancer center under a FRCP outbreak since 2019, reported an increased FRCP candidemia incidence in May 2020. Therefore, the purpose of this study was to investigate an inter-hospital candidemia outbreak caused by FRCP isolates during the first year of the COVID-19 pandemic in Brazil. C. parapsilosis bloodstream isolates obtained from the cancer (n = 35) and cardiology (n = 30) centers in 2020 were submitted to microsatellite genotyping and fluconazole susceptibility testing. The ERG11 gene of all isolates from the cardiology center was sequenced and compared to the corresponding sequences of the FRCP genotype responsible for the cancer center outbreak in 2019. Unprecedentedly, most of the FRCP isolates from the cardiology center presented the same genetic profile and Erg11-Y132F mutation detected in the strain that has been causing the persistent outbreak in the cancer center, highlighting the uninterrupted horizontal transmission of clonal isolates in our hospitals during the COVID-19 pandemic. (AU)

• Processo FAPESP: 18/19347-4 - Caracterização de mecanismos moleculares associados à resistência aos derivados azólicos em cepas clínicas de Trichosporon asahii
• Beneficiário: João Nóbrega de Almeida Júnior
• Modalidade de apoio: Bolsas no Exterior - Pesquisa
• Processo FAPESP: 18/15491-3 - Diversidade genética de Candida parapsilosis (sensu stricto): correlação de clusters emergentes com suscetibilidade a antifúngicos, formação de biofilme e virulência de isolados obtidos de Candidíase Invasiva em hospital terciário
• Beneficiário: Danilo Yamamoto Thomaz
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 21/00871-8 - Determinação do perfil de formação de biofilme de isolados de Candida parapsilosis resistentes ao fluconazol obtidos de um surto hospitalar de candidemia
• Beneficiário: Leidiane Barbosa Ribeiro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 19/17851-0 - Genotipagem por análise de microssatélites de isolados de Candida parapsilosis (sensu stricto) obtidos de casos de Candidíase Invasiva
• Beneficiário: Gabrielle Oliveira Marques Haddad Carvalho
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica