Transcriptomic analysis of cumulus cells shows altered pathways in patients with minimal and mild endometriosis

Autor(es)

Da Luz, Caroline Mantovani ; Da Broi, Michele Gomes ; Koopman, Larissa de Oliveira ; Placa, Jessica Rodrigues ; da Silva-Jr, Wilson Araujo ; Ferriani, Rui Alberto ; Meola, Juliana ; Navarro, Paula Andrea

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 12, n. 1, p. 9-pg., 2022-04-06.

Resumo

Endometriosis is a chronic inflammatory disorder that is highly associated with infertility. This association seems to be related to oocyte impairment, mainly in the initial stages of endometriosis (minimal and mild), where no distortions or adhesions are present. Nonetheless, invasive oocyte analyses are not routinely feasible; thus, indirect assessment of oocyte quality is highly desirable, and, in this context, cumulus cells (CCs) may be more suitable targets of analysis. CCs are crucial in oocyte development and could be used as an index of oocyte quality. Therefore, this prospective case-control study aimed to shed light on the infertility mechanisms of endometriosis I/II by analyzing the CCs' mRNA transcription profile (women with endometriosis I/II, n = 9) compared to controls (women with tubal abnormalities or male factor, n = 9). The transcriptomic analyses of CCs from patients with minimal and mild endometriosis revealed 26 differentially expressed genes compared to the controls. The enrichment analysis evidenced some altered molecular processes: Cytokine-cytokine receptor interactions, Chemokine signaling, TNF signaling, NOD-like receptor signaling, NF-kappa B signaling, and inflammatory response. With the exception of CXCL12, all enriched genes were downregulated in CCs from patients with endometriosis. These findings provide a significant achievement in the field of reproductive biology, directing future studies to discover biomarkers of oocyte quality in endometriosis. (AU)

• Processo FAPESP: 14/05878-7 - Perfil diferencial de expressão gênica em células do cumulus de mulheres inférteis com e sem endometriose submetidas à estimulação ovariana
• Beneficiário: Paula Andrea de Albuquerque Salles Navarro
• Modalidade de apoio: Auxílio à Pesquisa - Regular