The complete mitochondrial genome of carnivorous Genlisea tuberosa (Lentibulariaceae): Structure and evolutionary aspects

Autor(es)

Matos, Ramon Guedes ; Silva, Saura Rodrigues ; Plachno, Bartosz J. ; Adamec, Lubomir ; Michael, Todd P. ; Varani, Alessandro Mello ; Miranda, Vitor F. O.

• Tipo de documento: Artigo Científico
• Fonte: Gene; v. 824, p. 8-pg., 2022-05-25.

Resumo

Sequenced genomic data for carnivorous plants are scarce, especially regarding the mitogenomes (MTs) and further studies are crucial to obtain a better understanding of the topic. In this study, we sequenced and characterized the mitochondrial genome of the tuberous carnivorous plant Genlisea tuberosa, being the first of its genus to be sequenced. The genome comprises 729,765 bp, encoding 80 identified genes of which 36 are protein-coding, 40 tRNA, four rRNA genes, and three pseudogenes. An intronic region from the cox1 gene was identified that encodes an endonuclease enzyme that is present in the other sequenced species of Lentibulariaceae. Chloroplast genes (pseudogene and complete) inserted in the MT genome were identified, showing possible horizontal transfer between organelles. In addition, 50 pairs of long repeats from 94 to 274 bp are present, possibly playing an important role in the maintenance of the MT genome. Phylogenetic analysis carried out with 34 coding mitochondrial genes corroborated the positioning of the species listed here within the family. The molecular dynamism in the mitogenome (e.g. the loss or pseudogenization of genes, insertion of foreign genes, the long repeats as well as accumulated mutations) may be reflections of the carnivorous lifestyle where a significant part of cellular energy was shifted for the adaptation of leaves into traps molding the mitochondrial DNA. The sequence and annotation of G. tuberosa's MT will be useful for further studies and serve as a model for evolutionary and taxonomic clarifications of the group as well as improving our comprehension of MT evolution. (AU)

• Processo FAPESP: 20/11053-1 - Filogenômica de Utricularia (seção Utricularia: Lentibulariaceae): uma possível história de evolução reticulada e estado de conservação de um grupo de plantas carnívoras
• Beneficiário: Vitor Fernandes Oliveira de Miranda
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/02285-6 - Genômica comparativa e evolução molecular de plantas carnívoras do gênero Utricularia (Lentibulariaceae)
• Beneficiário: Saura Rodrigues da Silva
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado