Use of Photodynamic Therapy Associated with Antimicrobial Peptides for Bacterial Control: A Systematic Review and Meta-Analysis

Autor(es)

Dias, Luana Mendonca ; Ferrisse, Tulio Morandin ; Medeiros, Karine Sousa ; Cilli, Eduardo Maffud ; Pavarina, Ana Claudia

• Tipo de documento: Artigo Científico
• Fonte: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 23, n. 6, p. 14-pg., 2022-03-01.

Resumo

Considering the challenges related to antimicrobial resistance, other strategies for controlling infections have been suggested, such as antimicrobial photodynamic therapy (aPDT) and antimicrobial peptides (AMP). This study aims to perform a systematic review and meta-analysis to obtain evidence on the antimicrobial effectiveness of aPDT associated with AMP and establish in vitro knowledge on this topic for further study designs. The PubMed, Scopus, Web of Science, Science Direct, Scielo, and Cochrane Library databases were searched. Two independent and calibrated researchers (Kappa = 0.88) performed all the systematic steps according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). The odds ratio (OR) was used as the effect measure. The Peto method was used to perform the meta-analysis due to the sparse data. Twenty studies were included in the present review. The result was significant (OR = 0.14/p = 0.0235/I-squared = 0%), showing better outcomes of aPDT associated with peptides than those of aPDT alone for controlling the microbial load. Only 20% of the studies included evaluated this approach in a biofilm culture. Combined treatment with aPDT and AMP highly increased the ability of microbial reduction of Gram-positive and Gram-negative bacteria. However, additional blind studies are required to evaluate the efficacy of this therapy on microbial biofilms. (AU)

• Processo FAPESP: 21/06476-3 - Análise do peptídeo Temporin-SHa sobre biofilme de Candida albicans e células da cavidade oral
• Beneficiário: Karine Sousa Medeiros
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 21/01191-0 - Indução de tolerância e caracterização do biofime de Candida albicans submetido à terapia fotodinâmica antimicrobiana
• Beneficiário: Túlio Morandin Ferrisse
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 20/16227-8 - Influência da terapia fotodinâmica combinada com peptídeos antimicrobianos sobre biofilmes de Candida albicans e cultura 3D de células
• Beneficiário: Luana Mendonça Dias Santana
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs