| Texto completo | |
| Autor(es): |
José Hernandes-Lopes
;
Juliana Erika de Carvalho Teixeira Yassitepe
;
Alessandra Koltun
;
Laurens Pauwels
;
Viviane Cristina Heinzen da Silva
;
Ricardo Augusto Dante
;
Isabel Rodrigues Gerhardt
;
Paulo Arruda
Número total de Autores: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | GENETICS AND MOLECULAR BIOLOGY; v. 46, n. 1 2023-03-03. |
| Resumo | |
Abstract Recent advances in genome editing have enormously enhanced the effort to develop biotechnology crops for more sustainable food production. CRISPR/Cas, the most versatile genome-editing tool, has shown the potential to create genome modifications that range from gene knockout and gene expression pattern modulations to allele-specific changes in order to design superior genotypes harboring multiple improved agronomic traits. However, a frequent bottleneck is the delivery of CRISPR/Cas to crops that are less amenable to transformation and regeneration. Several technologies have recently been proposed to overcome transformation recalcitrance, including HI-Edit/IMGE and ectopic/transient expression of genes encoding morphogenic regulators. These technologies allow the eroding of the barriers that make crops inaccessible for genome editing. In this review, we discuss the advances in genome editing in crops with a particular focus on the use of technologies to improve complex traits such as water use efficiency, drought stress, and yield in maize. (AU) | |
| Processo FAPESP: | 16/23218-0 - Centro de Pesquisa em Genômica Aplicada às Mudanças Climáticas |
| Beneficiário: | Edi Lúcia Sartorato |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa Centros de Pesquisa em Engenharia |
| Processo FAPESP: | 18/06442-9 - Descoberta de novas quinases associadas à resposta ao estresse hídrico em milho |
| Beneficiário: | Viviane Cristina Heinzen da Silva |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 21/08486-6 - Transformação genética de milho e de Arabidopsis thaliana para avaliação funcional de genes para tolerância a estresses abióticos |
| Beneficiário: | Alessandra Koltun |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |