| Full text | |
| Author(s): |
José Hernandes-Lopes
;
Juliana Erika de Carvalho Teixeira Yassitepe
;
Alessandra Koltun
;
Laurens Pauwels
;
Viviane Cristina Heinzen da Silva
;
Ricardo Augusto Dante
;
Isabel Rodrigues Gerhardt
;
Paulo Arruda
Total Authors: 8
|
| Document type: | Journal article |
| Source: | GENETICS AND MOLECULAR BIOLOGY; v. 46, n. 1 2023-03-03. |
| Abstract | |
Abstract Recent advances in genome editing have enormously enhanced the effort to develop biotechnology crops for more sustainable food production. CRISPR/Cas, the most versatile genome-editing tool, has shown the potential to create genome modifications that range from gene knockout and gene expression pattern modulations to allele-specific changes in order to design superior genotypes harboring multiple improved agronomic traits. However, a frequent bottleneck is the delivery of CRISPR/Cas to crops that are less amenable to transformation and regeneration. Several technologies have recently been proposed to overcome transformation recalcitrance, including HI-Edit/IMGE and ectopic/transient expression of genes encoding morphogenic regulators. These technologies allow the eroding of the barriers that make crops inaccessible for genome editing. In this review, we discuss the advances in genome editing in crops with a particular focus on the use of technologies to improve complex traits such as water use efficiency, drought stress, and yield in maize. (AU) | |
| FAPESP's process: | 16/23218-0 - The Genomics for Climate Change Research Center |
| Grantee: | Edi Lúcia Sartorato |
| Support Opportunities: | Research Grants - Research Centers in Engineering Program |
| FAPESP's process: | 18/06442-9 - Discovering new kinases and receptor-like kinases associated with the response to drought stress in maize |
| Grantee: | Viviane Cristina Heinzen da Silva |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 21/08486-6 - Genetic transformation of maize and Arabidopsis thaliana for functional evaluation of genes aiming at tolerance to abiotic stresses |
| Grantee: | Alessandra Koltun |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |