Effects of BlueM® against Streptococcus mutans biofilm and its virulence gene expression

Autor(es)

Veronica Canela Estevam dos Santos ^[1] ; Patricia Milagros Maquera-Huacho ^[2] ; Maria Júlia Mancim Imbriani ; Vivian M. Tellaroli Rodrigues Minhaco ; Denise M. Palomari Spolidorio ^[5]

Afiliação do(s) autor(es)

^[1] São Paulo State University. School of Dentistry. Department of Physiology and Pathology - Brasil

^[2] São Paulo State University. School of Dentistry. Department of Physiology and Pathology - Brasil

^[5] São Paulo State University. School of Dentistry. Department of Physiology and Pathology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 34, n. 1, p. 19-28, 2023-03-06.

Resumo

Abstract This study evaluated the antimicrobial capacity of BlueM® mouthwash against the bacterium Streptococcus mutans and its influence on gbpA gene expression as well as its cytotoxic effect on fibroblast cells. BlueM® showed antimicrobial activity, with MIC and MBC values of 0.005% and 0.01%, respectively. The MBIC was 6.25% for S. mutans. CFU count and confocal microscopy revealed significant effect of BlueM® on S. mutans biofilm pre-formed on dentin surfaces. Interestingly, the analysis of gbpA gene expression indicated a decrease in gene expression after 15 min of treatment with BlueM® at a concentration of 25%. Moreover, BlueM® exhibited low levels of cytotoxicity. In conclusion, our results showed the antimicrobial effectiveness of BlueM® against S. mutans, its ability to modulate the expression of the gbpA gene and its low cytotoxicity. This study supports the therapeutic potential of BlueM® as an alternative agent for the control of oral biofilm. (AU)

• Processo FAPESP: 19/25534-4 - Efeito do oxigênio ativo sobre biofilme de Streptococcus mutans
• Beneficiário: Veronica Canela Estevam dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica