| Texto completo | |
| Autor(es): |
Cerni, Felipe
;
Oliveira, Isadora
;
Cordeiro, Francielle
;
Bordon, Karla
;
Ferreira, Isabela
;
Monteiro, Wuelton
;
Arantes, Eliane
;
Cunha, Thiago
;
Pucca, Manuela
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLoS Neglected Tropical Diseases; v. 17, n. 1, p. 14-pg., 2023-01-01. |
| Resumo | |
Scorpion sting envenomations (SSE) are feared by the intense pain that they produce in victims. Pain from SSE is triggered mainly by the presence of neurotoxins in the scorpion venom that modulates voltage-gated ion channels. In Brazil, SSE is mostly caused by Tityus serrulatus, popularly known as yellow scorpion. Here, we evaluated experimental spontaneous nociception induced by T. serrulatus venom as well as its isolated neurotoxins Ts1, Ts5, Ts6, Ts8, and Ts19 frag II, evidencing different degrees of pain behavior in mice. In addition, we developed a mice-derived polyclonal antibody targeting Ts5 able to neutralize the effect of this neurotoxin, showing that Ts5 presents epitopes capable of activating the immune response, which decreased considerably the nociception produced by the whole venom. This is the pioneer study to explore nociception using different classes of T. serrulatus neurotoxins on nociception (alpha-NaTx, beta-NaTx, alpha-KTx, and beta-KTx), targeting potassium and sodium voltage-gated channels, besides demonstrating that Ts5 plays an important role in the scorpion sting induced-pain. Author summaryThe Brazilian Tityus serrulatus scorpion envenoming is well recognized to be responsible for its painful sting. The pain reported by victims of T. serrulatus has shown variations, varying from mild to severe. However, little is known regarding the mechanisms involving the venom-derived toxins and their relationship a role in causing nociception. This research pioneer shows how each T. serrulatus classes of neurotoxins (Ts1: beta-NaTx; Ts5: alpha-NaTx; Ts6: alpha-KTx; Ts8 and Ts19: beta-KTx) are involved in the spontaneous nociception. The new knowledge may contribute to a better overall understanding of the mechanisms underlying T. serrulatus painful envenomings. (AU) | |
| Processo FAPESP: | 20/13176-3 - Descoberta de anticorpos monoclonais humanos (scFv) com reatividade cruzada e pH-dependentes para as metaloproteases de Bothrops spp |
| Beneficiário: | Isadora Sousa de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 19/10173-6 - Produção, modificação e caracterização de toxinas animais com potencial aplicação biotecnológica |
| Beneficiário: | Eliane Candiani Arantes Braga |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 13/08216-2 - CPDI - Centro de Pesquisa em Doenças Inflamatórias |
| Beneficiário: | Fernando de Queiroz Cunha |
| Modalidade de apoio: | Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs |