Inhibitory activity of S-PRG filler on collagen-bound MMPs and dentin matrix degradation

Autor(es)

Mendes Soares, Igor Paulino ; Anselmi, Caroline ; Guine, Isabela ; Fernandes, Lidia de Oliveira ; Barucci Araujo Pires, Maria Luiza ; de Souza Costa, Carlos Alberto ; Salles Scheffel, Debora Lopes ; Hebling, Josimeri

• Tipo de documento: Artigo Científico
• Fonte: Journal of Dentistry; v. 124, p. 7-pg., 2022-07-26.

Resumo

Objectives: To evaluate the inhibitory activity of an ion-releasing filler (S-PRG) eluate on dentin collagen-bound metalloproteinases (MMPs) and dentin matrix degradation. Methods: Dentin beams (5 x 2 x 0.5 mm) from human molars were completely demineralized to produce dentin matrix specimens. The dry mass was measured, and a colorimetric assay (Sensolyte) determined the initial total MMP activity to allocate the beams into four treatment groups (n = 10/group): 1) water for 1 min (negative control); 2) 2% chlorhexidine digluconate (CHX - inhibitor control) for 1 min; 3) S-PRG eluate for 1 min; 4) SPRG eluate for 30 min. After the treatments, the total MMP activity was reassessed. The specimens were stored in simulated body fluid (SBF) at 37 degrees C for up to 21 days. The dry mass was reassessed weekly. On day 7, the dentin matrix degradation was analyzed for the presence of collagen fragments (CF; Sirius Red) and hydroxyproline (Hyp) in the SBF. Statistical analyses were performed with ANOVA/Tukey, paired t-tests, and RM-ANOVA/Sidak (alpha = 5%). Results: S-PRG eluate exposure for 1 and 30 min reduced (p < 0.0001) MMP activity. S-PRG exposure for 30 min presented MMP activity inhibition equivalent to CHX (p = 0.061). S-PRG and CHX decreased CF (p <= 0.007) and Hyp (p < 0.046) release. After 21 days of storage, S-PRG-treated beams, regardless of exposure time, presented a reduced (p <= 0.017) mass loss, intermediate between CHX and control. Conclusion: Treating demineralized dentin with S-PRG eluate for 1 or 30 min reduced matrix-bound MMP activity and dentin matrix degradation for up to 21 days. Clinical significance: S-PRG filler may hinder the progression of dentin carious/erosive lesions and enhance the stabilization of dentin bonding interfaces. (AU)

• Processo FAPESP: 19/16473-1 - Scaffolds de nanofibras de policaprolactona-nano-hidroxiapatita para regeneração do complexo dentino-pulpar: síntese, caracterização e avaliação em cultura de dentes humanos
• Beneficiário: Josimeri Hebling Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular