| Texto completo | |
| Autor(es): Mostrar menos - |
de Lucas, Rosymar Coutinho
;
de Oliveira, Tassio Brito
;
Lima, Matheus Sanita
;
Pasin, Thiago Machado
;
de Almeida Scarcella, Ana Silvia
;
Costa Ribeiro, Liliane Fraga
;
Carvalho, Caio
;
de Lima Damasio, Andre Ricardo
;
Buckeridge, Marcos Silveira
;
Prade, Rolf Alexander
;
Segato, Fernando
;
Teixeira de Moraes Polizeli, Maria de Lourdes
Número total de Autores: 12
|
| Tipo de documento: | Artigo Científico |
| Fonte: | RENEWABLE ENERGY; v. 165, p. 10-pg., 2021-03-01. |
| Resumo | |
The plant cell wall is the most abundant carbon reservoir in nature and is a renewable source of biofuels. To break down this biomass and convert it into fermentable sugars, a set of multiple enzymes is needed. Here, we characterize the enzymatic repertoire necessary for the degradation of sugarcane bagasse "in natura" and pre-treated using Aspergillus clavatus as a model. 135 unique peptides were identified by Mass Spectrometry MS/MS. 23 of these proteins belong to classes of enzymes involved in biomass degradation and were differentially expressed on various substrates. Each pretreatment changed the sugarcane bagasse composition, which, in turn, led to the differential expression of A. clavatus holocellulases. The deconstruction of "in natura" bagasse demanded the largest set of enzymes due to the structural complexity of this material. Not only different sources of biomass but also different pretreatments of the same source will determine the enzymes required for the most efficient biomass conversion, avoiding the use of non-essential enzymes and consequent financial expense. Understanding A. clavatus nutritional strategies by proteomic analysis of secretome can improve the technology applied to biomass conversion and by-product synthesis. (C) 2020 Published by Elsevier Ltd. (AU) | |
| Processo FAPESP: | 10/52322-3 - Bioprospecção de fungos filamentosos produtores de holoenzimas com aplicação em biorefinaria |
| Beneficiário: | Maria de Lourdes Teixeira de Moraes Polizeli |
| Modalidade de apoio: | Auxílio à Pesquisa - Programa BIOTA - Regular |
| Processo FAPESP: | 17/09000-4 - Efeitos da temperatura e da deficiência hídrica, em cenário climático futuro, na comunidade de fungos em solo tropical e na seleção de linhagens produtoras de óxido nitroso |
| Beneficiário: | Tássio Brito de Oliveira |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 11/02169-7 - Aspergillus nidulans como modelo para expressão heteróloga de celulases e hemicelulases |
| Beneficiário: | André Ricardo de Lima Damasio |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |