Effects of different branched-chain amino acids supplementation protocols on the inflammatory response of LPS-stimulated RAW 264.7 macrophages

Autor(es)

Bonvini, Andrea ; Rogero, Marcelo Macedo ; Coqueiro, Audrey Yule ; Raizel, Raquel ; Bella, Leonardo Mendes ; Fock, Ricardo Ambrosio ; Borelli, Primavera ; Tirapegui, Julio

• Tipo de documento: Artigo Científico
• Fonte: Amino Acids; v. 53, n. 4, p. 11-pg., 2021-03-14.

Resumo

Although branched-chain amino acids (BCAA) are commonly used as a strategy to recover nutritional status of critically ill patients, recent findings on their role as immunonutrients have been associated with unfavorable outcomes, especially in obese patients. The present study aimed to explore the effects of different BCAA supplementation protocols in the inflammatory response of LPS-stimulated RAW 264.7 macrophages. Cell cultures were divided into five groups, with and without BCAA supplementation, (2 mmol/L of each amino acid). Then, cell cultures followed three different treatment protocols, consisting of a pretreatment (PT), an acute treatment (AT), and a chronic treatment (CT) with BCAA and LPS stimulation (1 mu g/mL). Cell viability was analyzed by MTT assay, NO production was assessed by the Griess reaction and IL-6, IL-10, TNF-alpha and PGE2 synthesis, was evaluated by ELISA. BCAA significantly increased cell viability in AT and CT protocols, and NO and IL-10 synthesis in all treatment protocols. IL-6 synthesis was only increased in PT and CT protocols. TNF-alpha and PGE2 synthesis were not altered in any of the protocols and groups. BCAA supplementation was able to increase both pro and anti-inflammatory mediators synthesis by RAW 264.7 macrophages, which was influenced by the protocol applied. Moreover, these parameters were significantly increased by isoleucine supplementation, highlighting a potential research field for future studies. (AU)

• Processo FAPESP: 16/04910-0 - Efeito da suplementação crônica com L-glutamina e L-alanina, nas formas livres ou como dipeptídeo, sobre parâmetros associados à fadiga em ratos submetidos ao treinamento resistido.
• Beneficiário: Julio Orlando Tirapegui Toledo
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/11360-6 - Efeitos dos aminoácidos de cadeia ramificada na resposta inflamatória induzida por lipopolissacarídeo (LPS) em macrófagos de linhagem celular RAW 264.7
• Beneficiário: Andrea Bonvini
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto